Arthrobacter phage Studio
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| Detailed Information for Phage Studio | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis NRRL B-4425 |
| Found By | Audrey Desjardins-Arturi |
| Year Found | 2025 |
| Location Found | Rockland, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 45.541552 N, 75.294449 W Map |
| Discovery Notes | Phage Studio was discovered from a soil sample collected on September 19th from a garden bed in the Rockland community garden. The sample site was located at 45.5415523, -75.2944494 with an ambient temperature of approximately 15 °C at the time of collection. Nanopore sequencing of the Studio genome produced 18 contigs, with the longest contig measuring 8,657 bp. BLASTn analysis of this contig revealed high-confidence matches (E-value = 0) to two Cluster FR Arthrobacter phages, AnnabelLee and Neuvillette, with over 80% coverage. The alignment to Neuvillette showed 92% identity across 5,700 bp with 0% gaps, while the alignment to AnnabelLee produced 91% identity across 5,700 bp, also with 0% gaps. These strong matches indicate that Studio shares close genomic similarity with both AnnabelLee and Neuvillette, supporting its placement within Cluster FR and suggesting a related genome structure. |
| Naming Notes | The name studio comes from my background as a competitive dancer and now a dance teacher. Dance is my passion and growing up I was always at the dance studio, thus why I chose that name. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 1244 |
| Genome length (bp) | 40828 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCTACGGCAT |
| GC Content | 58.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | FR |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members | |
| Annotating Institution | University of Ottawa |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | The isolated bacteriophage Studio produced small, clear plaques on Arthrobacter globiformis, a morphology that is generally suggestive of a lytic infection cycle. However, Studio did not show consistent plaque formation throughout the purification process, with plaque appearance varying noticeably across different host batches. |
| Number of Genes | 69 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewStudio_1 Studio_2 Studio_3 Studio_4 Studio_5 Studio_6 Studio_7 Studio_8 Studio_9 Studio_10 Studio_11 Studio_12 Studio_13 Studio_14 Studio_15 Studio_16 Studio_17 Studio_18 Studio_19 Studio_20 Studio_21 Studio_22 Studio_23 Studio_24 Studio_25 Studio_26 Studio_27 Studio_28 Studio_29 Studio_30 Studio_31 Studio_32 Studio_33 Studio_34 Studio_35 Studio_36 Studio_37 Studio_38 Studio_39 Studio_40 Studio_41 Studio_42 Studio_43 Studio_44 Studio_45 Studio_46 Studio_47 Studio_48 Studio_49 Studio_50 Studio_51 Studio_52 Studio_53 Studio_54 Studio_55 Studio_56 Studio_57 Studio_58 Studio_59 Studio_60 Studio_61 Studio_62 Studio_63 Studio_64 Studio_65 Studio_66 Studio_67 Studio_68 Studio_69 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
