Streptomyces phage Success
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Detailed Information for Phage Success
Discovery Information
Isolation HostStreptomyces lividans JI 1326
Found ByJay Msuya and Mason Roth
Year Found2021
Location FoundSt. Louis, MO United States
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature31°C
GPS Coordinates38.643333 N, 90.315556 W Map
Discovery NotesWas Found outside of Wheeler House on the Washington University in St. Louis campus
Naming NotesIt took many attempts to isolate a phage, so the name "Success" speaks to the feeling of accomplishment felt after a lot of failure.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 7, 2022
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage326
Genome length (bp)57163
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGCAGGGGGCG
GC Content61.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterSingleton
Subcluster--
Cluster Life CycleUnknown
Lysogeny NotesAppears to be negative for lysogeny based on plaque morphology. Not definitive.
Other Cluster Members
Annotating InstitutionWashington University in St. Louis
Annotation StatusIn GenBank
Plaque NotesAppear to be clear and rough around the edges. Approximately 1.5mm in diameter.
MorphotypeSiphoviridae
Number of Genes84
Number of tRNAs8
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Success_1
(kinase)

Success_2

Success_3
(oxidoreductase)

Success_4

Success_5

Success_6
(helix-turn-helix DNA binding domain protein)

Success_7

Success_8

Success_9
(helix-turn-helix DNA binding domain protein)

Success_10
(metalloprotease)

Success_11
(tyrosine homologous recombinase)

Success_12
(helix-turn-helix DNA binding domain protein)

Success_13
(deoxycytidylate deaminase)

Success_14

Success_15

Success_16
(DprA-like DNA processing chain A)

Success_17

Success_18

Success_19
(DNA polymerase I)

Success_20
(DNA methyltransferase)

Success_21
(ThyX-like thymidylate synthase)

Success_22

Success_23
(ribonucleotide reductase)

Success_24
(DNA binding protein)

Success_25
(metallophosphoesterase)

Success_26

Success_27

Success_28

Success_29
(DNA primase)

Success_30
(DNA primase)

Success_31

Success_32

Success_33
(endonuclease VII)

Success_34
(holliday junction resolvase)

Success_35
(DnaB-like dsDNA helicase)

Success_36
(Cas4 exonuclease)

Success_37

Success_38
(helix-turn-helix DNA binding domain protein)

Success_39
(HNH endonuclease)

Success_40 — tRNA
(tRNA-Phe(gaa))

Success_41 — tRNA
(tRNA-Ile(gat))

Success_42 — tRNA
(tRNA-Glu(ttc))

Success_43 — tRNA
(tRNA-Asp(gtc))

Success_44 — tRNA
(tRNA-Asn(gtt))

Success_45 — tRNA
(tRNA-Lys(ctt))

Success_46 — tRNA
(tRNA-Trp(cca))

Success_47 — tRNA
(tRNA-Pro(tgg))

Success_48

Success_49
(terminase small subunit)

Success_50
(terminase large subunit)

Success_51
(portal protein)

Success_52
(scaffolding protein)

Success_53
(major capsid protein)

Success_54
(head-to-tail adaptor)

Success_55
(head-to-tail stopper)

Success_56

Success_57
(tail terminator)

Success_58
(major tail protein)

Success_59
(tail assembly chaperone)

Success_60
(tail assembly chaperone)

Success_61
(tape measure protein)

Success_62
(minor tail protein)

Success_63
(minor tail protein)

Success_64
(minor tail protein)

Success_65
(minor tail protein)

Success_66

Success_67
(endolysin)

Success_68

Success_69

Success_70

Success_71
(Lsr2-like DNA bridging protein)

Success_72

Success_73

Success_74
(DNA binding protein)

Success_75

Success_76

Success_77

Success_78
(DNA binding protein)

Success_79

Success_80

Success_81

Success_82

Success_83

Success_84

Success_85

Success_86

Success_87

Success_88

Success_89

Success_90

Success_91

Success_92

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOP751148
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 1.22x10^9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload