Mycobacterium phage TSine
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| Detailed Information for Phage TSine | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Tate Sine |
| Year Found | 2024 |
| Location Found | Bowling Green, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | Unavailable |
| Discovery Notes | I discovered this phage In the front yard of my house. The site is surrounded by vegetation including grass, trees, flowers, and bushes. The soil was moist as it had rained the evening before collecting the sample. The site is isolated from human foot traffic as it is in the middle of my front yard. |
| Naming Notes | My nickname has always been TSine. So I decided to name my phage after my life-long nickname! |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | I tested to see if my lysogen was immune by observing dilutions on a lawn of my one plate lysate and a control lawn. Normally I wouldn’t see any spots on my streak plate confirming the lysogen was immune. However, since I am seeing spots this means that I could have picked up a completely different colony while streaking. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The morphology of the phage was tested using the turbidity test as well as the temperature test. The turbidity test required the production of a streak plate. After streaking the plate with the one-plate lysate from a turbid plaque it was allowed time to incubate. Figure The results of the turbidity test displayed formation of many turbid plaques. The temperature test results showed phages present after incubation at 30 degrees celsius while none showed up at 37 degrees celsius. This shows that phage growth is optimal at 30 degrees while 37 degrees is not a respectable temperature for phage growth. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |