Mycobacterium phage TechDeck
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| Detailed Information for Phage TechDeck | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Nikolas Stawicki |
| Year Found | 2023 |
| Location Found | Bowling Green, KY United States of America |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.944964 N, 86.419455 W Map |
| Discovery Notes | This was found in soil that was in a mulch bed in front of my apartment. |
| Naming Notes | I named this phage after a popular toy I had growing up |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | To determine the phenotypic morphology of the phage TechDeck, we had to see if there was any difference when the phage was incubated at different temperatures. We incubated the phage on 2 separate spot plates, one at 30ºC, our normal incubation temperature, and one at 37ºC. After incubation, it was determined that the morphology had not changed at all between the different temperatures. The spot plates exhibited mostly clear lysing of the infected area, with more turbid mutants appearing towards the outermost boundary of the spot |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | It has always been a fairly clear plaque morphology, but as I further diluted the phages and plated them, the plaques have turned more turbid. The average size of either the clear or turbid plaques were all around 2mm. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 1.35 x 10^10 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
