Mycobacterium phage TiPumba
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| Detailed Information for Phage TiPumba | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Kelsey Schaaf, Sarah Muhr |
| Year Found | 2024 |
| Location Found | Spokane, WA United States |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 22°C |
| GPS Coordinates | 47.6685 N, 117.40254 W Map |
| Discovery Notes | The soil was darker in color and slightly damp, as it had rained the night before. We extracted our sample from under and acorn tree on August 27th, 2024. |
| Naming Notes | Our lab section discussed naming our phages based off of Disney characters, so my partner and I came up with a mixed name from Timon and Pumba in the Lion King. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaques are uniformly distributed across the petri dish, presenting as small round clearings. The plaques are densely packed and well-defined, indicating successful lysis of host cells. However as we were analyzing our spot-titer, we found no plaques. From then, all of our petri dishes (including our full plate titers, another spot titer attempt, and the spot titers that contain different bacterial hosts and different temperatures) had no plaques. We also did not receive a TEM image because there was no detectable phage in our sample. Throughout the rest of our experimentations, we found no evidence of phage. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
