Mycobacterium phage Totoro
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| Detailed Information for Phage Totoro | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Angela Albrecht |
| Year Found | 2015 |
| Location Found | Northampton, PA USA |
| Finding Institution | Lehigh University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | -7°C |
| GPS Coordinates | 40.755 N, 75.479 E Map |
| Discovery Notes | Phage was discovered from composted soil sample in Northampton, PA at -7 degrees Celcius. Sample was collected from soil 18 cm deep. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | After 24 hours of incubation, plaques are turbid, roughly 3 mm in diameter with small clearing in center (similar to a dot) less than .5 mm. After 48 hours of incubation plaques grew to 3.5-4.0 mm in size. The clearing in the center became .5 mm larger in addition. The phage is a siphoviridae with an icosahedral head, although it appears to have a spherical shape. At the bottom of its tail is a round structure where the phage has its landing gear. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2.72 x 10^9 |
| Date of SEA Lysate Titering | Mar 24, 2015 |
| Pitt Freezer Box# | 15 |
| Pitt Freezer Box Grid# | H4 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |