Mycobacterium phage Totoro
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Detailed Information for Phage Totoro | |
Discovery Information | |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Angela Albrecht |
Year Found | 2015 |
Location Found | Northampton, PA USA |
Finding Institution | Lehigh University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | -7°C |
GPS Coordinates | 40.755 N, 75.479 E Map |
Discovery Notes | Phage was discovered from composted soil sample in Northampton, PA at -7 degrees Celcius. Sample was collected from soil 18 cm deep. |
Sequencing Information | |
Sequencing Complete? | No |
Genome length (bp) | Unknown |
Character of genome ends | Unknown |
Fasta file available? | No |
Characterization | |
Cluster | Unclustered |
Subcluster | -- |
Annotating Institution | Unknown or unassigned |
Annotation Status | Not sequenced |
Plaque Notes | After 24 hours of incubation, plaques are turbid, roughly 3 mm in diameter with small clearing in center (similar to a dot) less than .5 mm. After 48 hours of incubation plaques grew to 3.5-4.0 mm in size. The clearing in the center became .5 mm larger in addition. The phage is a siphoviridae with an icosahedral head, although it appears to have a spherical shape. At the bottom of its tail is a round structure where the phage has its landing gear. |
Morphotype | Siphoviridae |
Has been Phamerated? | No |
Publication Info | |
Uploaded to GenBank? | No |
GenBank Accession | None yet |
Refseq Number | None yet |
Archiving Info | |
Archiving status | Archived |
SEA Lysate Titer | 2.72 x 10^9 |
Date of SEA Lysate Titering | Mar 24, 2015 |
Pitt Freezer Box# | 15 |
Pitt Freezer Box Grid# | H4 |
Available Files | |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |