The Actinobacteriophage Database

Gordonia phage VanLee

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Detailed Information for Phage VanLee
Discovery Information
Isolation Host	Gordonia rubripertincta NRRL B-16540
Found By	Vanessa Franco and Kaylee Barnhill
Year Found	2020
Location Found	Tampa, FL United States of America
Finding Institution	University of South Florida
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	31°C
GPS Coordinates	28.063333 N, 82.411389 W Map
Discovery Notes	Isolated from a moist soil sample from Tampa, Florida (28.063333N, 82.411389W. VanLee has host range and can infect Gordonia terrae with the same efficiency as G. rubripertincta
Naming Notes	Naming is based on the two students who found and characterized the phage VANessa and kayLEE.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Dec 18, 2020
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina MiSeq
Read Type	Single-end reads
Read Length	150 bp
Approximate Shotgun Coverage	1488
Genome length (bp)	84560
Character of genome ends	Circularly Permuted
GC Content	67.8%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	KA
Subcluster	--
Cluster Life Cycle	Unknown
Other Cluster Members	Click to View Colossa VanLee
Annotating Institution	University of South Florida
Annotation Status	In GenBank
Morphotype	Siphoviridae
Number of Genes	164
Number of tRNAs	1
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View VanLee_1 (terminase small subunit) VanLee_2 (helix-turn-helix DNA-binding domain protein) VanLee_3 (terminase large subunit) VanLee_4 (portal protein) VanLee_5 VanLee_6 (MuF-like minor capsid protein) VanLee_7 (scaffolding protein) VanLee_8 (major capsid protein) VanLee_9 VanLee_10 (head-to-tail adaptor) VanLee_11 (head-to-tail stopper) VanLee_12 VanLee_13 (tail terminator) VanLee_14 (major tail protein) VanLee_15 VanLee_16 (tail assembly chaperone) VanLee_17 (tail assembly chaperone) VanLee_18 (tape measure protein) VanLee_19 (minor tail protein) VanLee_20 (minor tail protein) VanLee_21 VanLee_22 (minor tail protein) VanLee_23 VanLee_24 VanLee_25 (minor tail protein) VanLee_26 VanLee_27 VanLee_28 (lysin A, N-acetylmuramoyl-L-alanine amidase domain) VanLee_29 VanLee_30 VanLee_31 VanLee_32 (tyrosine integrase) VanLee_33 (metalloprotease) VanLee_34 (Cro protein) VanLee_35 (immunity repressor) VanLee_36 (Cas4 family exonuclease) VanLee_37 (RecT-like DNA pairing protein) VanLee_38 VanLee_39 VanLee_40 (excise) VanLee_41 VanLee_42 (ssDNA binding protein) VanLee_43 VanLee_44 (RepA-like replication initiator) VanLee_45 (RepA-like replication initiator) VanLee_46 (DnaB-like dsDNA helicase) VanLee_47 VanLee_48 VanLee_49 VanLee_50 VanLee_51 VanLee_52 VanLee_53 VanLee_54 VanLee_55 VanLee_56 VanLee_57 VanLee_58 VanLee_59 VanLee_60 VanLee_61 VanLee_62 VanLee_63 VanLee_64 VanLee_65 VanLee_66 VanLee_67 VanLee_68 VanLee_69 VanLee_70 VanLee_71 VanLee_72 VanLee_73 VanLee_74 VanLee_75 VanLee_76 (helicase) VanLee_77 VanLee_78 VanLee_79 VanLee_80 VanLee_81 VanLee_82 VanLee_83 VanLee_84 VanLee_85 VanLee_86 VanLee_87 (DnaQ-like DNA polymerase III subunit) VanLee_88 (nuclease) VanLee_89 VanLee_90 VanLee_91 (PnuC-like nicotinamide riboside transporter) VanLee_92 (helix-turn-helix DNA-binding domain protein) VanLee_93 VanLee_94 VanLee_95 VanLee_96 VanLee_97 VanLee_98 VanLee_99 VanLee_100 VanLee_101 (HicB-like antitoxin) VanLee_102 VanLee_103 (CobT-like cobalamin biosynthesis protein) VanLee_104 (lysin B) VanLee_105 VanLee_106 VanLee_107 (helix-turn-helix DNA-binding domain protein) VanLee_108 VanLee_109 VanLee_110 (nucleotidyltransferase) VanLee_111 VanLee_112 (DnaQ-like DNA polymerase III subunit) VanLee_113 VanLee_114 (polynucleotide kinase) VanLee_115 VanLee_116 (HNH endonuclease) VanLee_117 VanLee_118 VanLee_119 VanLee_120 VanLee_121 VanLee_122 (RuvC-like resolvase) VanLee_123 (HicA-like toxin) VanLee_124 (ribbon-helix-helix DNA binding domain protein) VanLee_125 — tRNA (tRNA-Arg(tct)) VanLee_126 (Ro-like RNA binding protein) VanLee_127 VanLee_128 VanLee_129 VanLee_130 (acetyltransferase) VanLee_131 VanLee_132 (DNA binding protein) VanLee_133 VanLee_134 VanLee_135 VanLee_136 VanLee_137 VanLee_138 VanLee_139 VanLee_140 VanLee_141 VanLee_142 VanLee_143 VanLee_144 VanLee_145 VanLee_146 VanLee_147 VanLee_148 VanLee_149 VanLee_150 VanLee_151 VanLee_152 VanLee_153 VanLee_154 VanLee_155 VanLee_156 VanLee_157 VanLee_158 VanLee_159 VanLee_160 VanLee_161 VanLee_162 VanLee_163 VanLee_164 (PAPS reductase-like domain protein) VanLee_165
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MZ028627
Refseq Number	None yet
Published in Paper	Franco et al 2021, Microbiology Resource Announcements: Genome Sequence of VanLee, a Singleton Actinobacteriophage That Infects Multiple Gordonia Strains
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	118
Pitt Freezer Box Grid#	F1
Available Files
Plaque Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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