| Detailed Information for Phage Vondra | |
| Discovery Information | |
| Isolation Host | Streptomyces sanglieri UNT16F27A |
| Found By | Jessica Vondra and Morgan Stovall |
| Year Found | 2019 |
| Location Found | Denton, TX United States |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.212232 N, 97.1485 W Map |
| Discovery Notes | I collected Vondra from the base of a tree located in front of the Life Science Complex B at the University of North Texas at 2:42 (14:42). I dug down about 3 centimeters and collected soil in a 15ml Conical Tube. The color was a light brown and had a very dry and sandy texture (there were little twigs mixed in). The outside temperature was 95°F |
| Naming Notes | I named the phage after my last name. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 17, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1550 |
| Genome length (bp) | 37129 |
| Character of genome ends | Circularly Permuted |
| GC Content | 71.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BP |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Lysogeny Notes | We performed a Lysogeny Culture Test. Vondra did not infect the lysogen, therefore Vondra is a Temperate Phage. |
| Other Cluster Members |
Click to View |
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | Vondra is a cloudy plaque with a diameter of 0.5 millimeters. |
| Number of Genes | 57 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewVondra_1 Vondra_2 Vondra_3 Vondra_4 Vondra_5 Vondra_6 Vondra_7 Vondra_8 Vondra_9 Vondra_10 Vondra_11 Vondra_12 Vondra_13 Vondra_14 Vondra_15 Vondra_16 Vondra_17 Vondra_18 Vondra_19 Vondra_20 Vondra_21 Vondra_22 Vondra_23 Vondra_24 Vondra_25 Vondra_26 Vondra_27 Vondra_28 Vondra_29 Vondra_30 Vondra_31 Vondra_32 Vondra_33 Vondra_34 Vondra_35 Vondra_36 Vondra_37 Vondra_38 Vondra_39 Vondra_40 Vondra_41 Vondra_42 Vondra_43 Vondra_44 Vondra_45 Vondra_46 Vondra_47 Vondra_48 Vondra_49 Vondra_50 Vondra_51 Vondra_52 Vondra_53 Vondra_54 Vondra_55 Vondra_56 Vondra_57 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT451981 |
| Refseq Number | None yet |
| Published in Paper | Nath et al 2022, Microbiology Resource Announcements: Complete Genome Sequences of the Novel Cluster BP Phages Infecting Streptomyces sanglieri, AxeJC, Cumberbatch, Eastland, Eklok, HFrancette, Ignacio, Piccadilly, and Vondra |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.6 x 10^9pfu/mL |
| Pitt Freezer Box# | 104 |
| Pitt Freezer Box Grid# | D6 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
