Mycobacterium phage WhyZach
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Detailed Information for Phage WhyZach
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJosh and Ethan
Year Found2017
Location FoundSpokane, WA USA
Finding InstitutionGonzaga University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature22°C
GPS Coordinates47.667784 N, 117.403439 W Map
Naming NotesThis phage is named after Gonzaga basketball player Zach Collins. He could potentially be leaving Gonzaga if he gets drafted for the NBA this year, but we really don't want him to do that, so WhyZach?
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Lysogeny NotesWhen examining the restriction enzymes that reacted with our phage DNA via gel electrophoresis, the only restriction enzyme to cut the phage's DNA was SalI. SalI only produced cuts in the DNA at approximately 6,000 base pairs and below. There was a high concentration of phage DNA in the samples with the following restriction enzymes: ClaI, EcoRI, HindIII, and SalI. BamHI and HaeIII had a lower concentration of phage DNA due to them having darker columns in the gel.
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesoWhen looking at our TEM image, Our phage has a small head (50 nm) and a long tail (220.83 nm), totaling 270.83 nm. It appears to be part of the siphoviridae family due to its long tail.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 65
Pitt Freezer Box Grid# G6
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload