| Detailed Information for Phage Wishmaker | 
	
	
		| Discovery Information | 
		| Isolation Host | Mycobacterium smegmatis mc²155 | 
		
		
		
		
		| Found By | Gabriela Mendez | 
		| Year Found | 2019 | 
		| Location Found | Harrisonburg, VA  United States | 
		| Finding Institution | James Madison University | 
		| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science | 
		| From enriched soil sample? | Unknown | 
		| Isolation Temperature | Not entered | 
		| GPS Coordinates | 38.0 N, 78.0 W  Map | 
		| Discovery Notes | Tube of phage solution was found in bioscience building but the origins of the actual phage remain unknown. | 
		| Naming Notes | Title of one of my favorite episodes in my favorite TV show | 
		
                
		| Sequencing Information | 
		| Sequencing Complete? | Yes | 
		| Date Sequencing Completed | Feb 16, 2023 | 
		| Sequencing Facility | Pittsburgh Bacteriophage Institute | 
		| Shotgun Sequencing Method | Illumina | 
		| Sequencer Used | Illumina MiSeq | 
		| Read Type | Single-end reads | 
		| Read Length | 150 bp | 
		| Approximate Shotgun Coverage | 1280 | 
		
		| Genome length (bp) | 53746 | 
		| Character of genome ends | 3' Sticky Overhang | 
		| Overhang Length | 14 bases | 
		| Overhang Sequence | TAAGCCGCGCGGTA | 
		
		
		| GC Content | 67.5% | 
		
		| Fasta file available? | Yes: Download fasta file | 
		| Characterization | 
		| Cluster | Q | 
		| Subcluster | -- | 
                | Cluster Life Cycle | Temperate | 
		
		| Other Cluster Members | 
                
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		| Annotating Institution | James Madison University | 
                | Annotation Status | In GenBank | 
		
		| Morphotype | Siphoviridae | 
		| Number of Genes | 78 | 
		| Number of tRNAs | 0 | 
		| Number of tmRNAs | 0 | 
		| Has been Phamerated? | Yes | 
		| Gene List | 
			
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		| Submitted Minimal DNA Master File | Download | 
		
		
		| Publication Info | 
		| Uploaded to GenBank? | Yes | 
		| GenBank Accession | PP208933 | 
		| Refseq Number | None yet | 
		
        
        | Archiving Info | 
        
        
          | Archiving status | 
          Not in Pitt Archives | 
        
        
        
        
        
        
        
		
		| Available Files | 
		
		
		
		
		
		
		| GenBank File for Phamerator | Download |