Arthrobacter phage YoshiYama
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| Detailed Information for Phage YoshiYama | |
| Discovery Information | |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Theophilus Sim, Teams Lysis League, Phinest Phour |
| Year Found | 2025 |
| Location Found | Chatsworth, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.260981 N, 118.578788 W Map |
| Discovery Notes | Dry soil with a mix of some roots. Found next to the “Splash Pad” in Mason park. Depth from which sample was taken (in cm): 6 cm Air temperature if sample collected outdoors (in degrees Celsius): 22oC Host-Enrichment using Arthrobacter sulfureus, Arthrobacter sp., A. atrocyaneus, and Arthrobacter globiformis. The soil sample containing the phage was incubated at 30 degrees Celsius for 15 hours with the host bacteria mixture. The mixture was then plated and incubated for 30 degrees Celsius for 48 hours to elicit plaque formation for observation. AL Quarter and Lab section: Instructor: Dr. Tejas Bouklas Teaching Assistant: Sasha Xu Undergraduate Assistant(s): Chantal, Sabrina Team #1 Name: Lysis League Team #1 Members: Ava Soliman, Theophilus Sim, Kailyn Kuo, and Kiara Mauro Team #2 Name: Phinest Phour Team #2 Members: Cian Cox, Renee Chowdhry, Gabriel Valenzuela, Jonny Zhao |
| Naming Notes | named after Yoshinobu Yamamoto, the MVP starting pitcher for the Dodgers’ win in the 2025 World Series |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 22, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 582 |
| Genome length (bp) | 58272 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGGCCT |
| GC Content | 45.2% |
| Fasta file available? | No |
| Characterization | |
| Cluster | AM |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | Fuzzy borders, turbidity Description of plaque morphology: Turbid, bulls-eye Average size (and size range) diameter in mm: 4mm |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
