| Detailed Information for Phage YoshiYama | |
| Discovery Information | |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Theophilus Sim, Teams Lysis League, Phinest Phour |
| Year Found | 2025 |
| Location Found | Chatsworth, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.260981 N, 118.578788 W Map |
| Discovery Notes | Dry soil with a mix of some roots. Found next to the “Splash Pad” in Mason park. Depth from which sample was taken (in cm): 6 cm Air temperature if sample collected outdoors (in degrees Celsius): 22oC Host-Enrichment using Arthrobacter sulfureus, Arthrobacter sp., A. atrocyaneus, and Arthrobacter globiformis. The soil sample containing the phage was incubated at 30 degrees Celsius for 15 hours with the host bacteria mixture. The mixture was then plated and incubated for 30 degrees Celsius for 48 hours to elicit plaque formation for observation. AL Quarter and Lab section: Instructor: Dr. Tejas Bouklas Teaching Assistant: Sasha Xu Undergraduate Assistant(s): Chantal, Sabrina Team #1 Name: Lysis League Team #1 Members: Ava Soliman, Theophilus Sim, Kailyn Kuo, and Kiara Mauro Team #2 Name: Phinest Phour Team #2 Members: Cian Cox, Renee Chowdhry, Gabriel Valenzuela, Jonny Zhao |
| Naming Notes | named after Yoshinobu Yamamoto, the MVP starting pitcher for the Dodgers’ win in the 2025 World Series |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 22, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 582 |
| Genome length (bp) | 58272 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGGCCT |
| GC Content | 45.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AM |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | Fuzzy borders, turbidity Description of plaque morphology: Turbid, bulls-eye Average size (and size range) diameter in mm: 4mm |
| Morphotype | Siphoviridae |
| Number of Genes | 96 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewYoshiYama_1 YoshiYama_2 YoshiYama_3 YoshiYama_4 YoshiYama_5 YoshiYama_6 YoshiYama_7 YoshiYama_8 YoshiYama_9 YoshiYama_10 YoshiYama_11 YoshiYama_12 YoshiYama_13 YoshiYama_14 YoshiYama_15 YoshiYama_16 YoshiYama_17 YoshiYama_18 YoshiYama_19 YoshiYama_20 YoshiYama_21 YoshiYama_22 YoshiYama_23 YoshiYama_24 YoshiYama_25 YoshiYama_26 YoshiYama_27 YoshiYama_28 YoshiYama_29 YoshiYama_30 YoshiYama_31 YoshiYama_32 YoshiYama_33 YoshiYama_34 YoshiYama_35 YoshiYama_36 YoshiYama_37 YoshiYama_38 YoshiYama_39 YoshiYama_40 YoshiYama_41 YoshiYama_42 YoshiYama_43 YoshiYama_44 YoshiYama_45 YoshiYama_46 YoshiYama_47 YoshiYama_48 YoshiYama_49 YoshiYama_50 YoshiYama_51 YoshiYama_52 YoshiYama_53 YoshiYama_54 YoshiYama_55 YoshiYama_56 YoshiYama_57 YoshiYama_58 YoshiYama_59 YoshiYama_60 YoshiYama_61 YoshiYama_62 YoshiYama_63 YoshiYama_64 YoshiYama_65 YoshiYama_66 YoshiYama_67 YoshiYama_68 YoshiYama_69 YoshiYama_70 YoshiYama_71 YoshiYama_72 YoshiYama_73 YoshiYama_74 YoshiYama_75 YoshiYama_76 YoshiYama_77 YoshiYama_78 YoshiYama_79 YoshiYama_80 YoshiYama_81 YoshiYama_82 YoshiYama_83 YoshiYama_84 YoshiYama_85 YoshiYama_86 YoshiYama_87 YoshiYama_88 YoshiYama_89 YoshiYama_90 YoshiYama_91 YoshiYama_92 YoshiYama_93 YoshiYama_94 YoshiYama_95 YoshiYama_96 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
