Arthrobacter phage YoshiYama
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Detailed Information for Phage YoshiYama
Discovery Information
Isolation HostArthrobacter sp. 68b
Found ByTheophilus Sim, Teams Lysis League, Phinest Phour
Year Found2025
Location FoundChatsworth, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates34.260981 N, 118.578788 W Map
Discovery NotesDry soil with a mix of some roots. Found next to the “Splash Pad” in Mason park.
Depth from which sample was taken (in cm): 6 cm
Air temperature if sample collected outdoors (in degrees Celsius): 22oC

Host-Enrichment using Arthrobacter sulfureus, Arthrobacter sp., A. atrocyaneus, and Arthrobacter globiformis. The soil sample containing the phage was incubated at 30 degrees Celsius for 15 hours with the host bacteria mixture. The mixture was then plated and incubated for 30 degrees Celsius for 48 hours to elicit plaque formation for observation.

Additional information of interest or note:
AL Quarter and Lab section:
Instructor: Dr. Tejas Bouklas
Teaching Assistant: Sasha Xu
Undergraduate Assistant(s): Chantal, Sabrina
Team #1 Name: Lysis League
Team #1 Members: Ava Soliman, Theophilus Sim, Kailyn Kuo, and Kiara Mauro
Team #2 Name: Phinest Phour
Team #2 Members: Cian Cox, Renee Chowdhry, Gabriel Valenzuela, Jonny Zhao
Naming Notesnamed after Yoshinobu Yamamoto, the MVP starting pitcher for the Dodgers’ win in the 2025 World Series
Sequencing Information
Sequencing Complete?No
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesFuzzy borders, turbidity
Description of plaque morphology (e.g., halos, clear or turbid): Turbid, bulls-eye
Average size (and size range) diameter in mm: 4mm
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload