Characterization: Protocols and Information
Characterization
Characterization of phages can mean many things. You will find more explorations for characterizations under the heading "Further Explorations". Included here are restriction digest and electron microscopy protocols.| Protocol Title | Description/Goal |
|---|---|
| Restriction Enzyme Digest Details Download pdf |
Create a “Phage Phingerprint” to compare a phage’s DNA to that of other phages/clusters by digesting it with several restriction enzymes, and then running those digests on an agarose gel. |
| Phage Sample Preparation for Electron Microscopy Details Download pdf |
To prepare phage sample for electron microscopy. |
| Electron Microscopy Grid Sample Preparation Details Download pdf |
To prepare (glow-discharge) grids for sample application. |
| Making Thumbnails for PhagesDB Details Download pdf |
Describes how to select, crop, and prepare good pictures for use as thumbnail images on PhagesDB.org. |