Recently Added Phages

KMAprongs Klimt Lukekane BadBB Buck34

Recently Modified Phages

Lollipop1437 JoieB VA6 Opie Mollymur

Recently Finished Phages

VA6 (A2) ANI8 (A2) JF2 (A3) JF4 (A3) MK4 (A3)
Extraction: Protocols and Information
Extraction

Extraction

The methods used in our lab for extracting clean DNA for sequencing, restriction digests, and further investigations are described here. It is important to know the concentration of phage (titer) before starting.
Protocol TitleDescription/Goal
PCI/SDS DNA Extraction
Details
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To extract a high yield, clean DNA sample from 1 mL high titer phage lysate.
PCI DNA Extraction
Details
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To extract a high yield, clean DNA sample from 15 mL high titer phage lysate.
Small-Scale Phage Concentration
Details
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To concentrate a phage sample using a small-scale prep.
Phage DNA Extraction Using the Promega Clean-Up Kit
Details
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Extract phage genomic DNA using the Promega DNA Clean-Up Kit.