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Gordonia phage Kiko
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Detailed Information for Phage Kiko
Discovery Information
Isolation HostGordonia terrae 3612
Found ByMira Patel
Year Found2017
Location FoundPittsburgh, PA United States
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.44555 N, 79.9561 W Map
Discovery NotesKiko was collected from soil on 1/9/17. On that day, the temperature was -3.9oC, and it was windy with a little snowfall. The soil was transported for approximately 10 minutes before it was brought to the laboratory. The soil was covered with snow and mulch on the top. The bottom layer of soil which was collected was softer.
Naming NotesKiko was the name of my pet bird.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 22, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2706
Genome length (bp)44268
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCCTCGGTCA
GC Content66.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterDB
Subcluster--
Other Cluster Members
Plaque NotesPlaques are mostly small and round, 1 mm in diameter. Some of the plaques do seem to have a tear-drop extension on it. The plaques are clustered together near one end of the plate.
There was a little size discrepancy of the plaques on my original plaque purifications.
The slight difference between the plaques is because Kiko is affected by direction. Since the bench is not exactly leveled, it would be microscopically deeper at one point; this difference means a lot to a phage because at the microscopic level a phage is much more affected. The difference in level effects the top agar and host cells when they are cooling on the plate. One end of the lawn would be slightly deeper than the other. There are smaller plaques where the agar is deeper because the phage did not diffuse as much.
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload