Gordonia phage Margaret
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Detailed Information for Phage Margaret
Discovery Information
Isolation HostGordonia terrae 3612
Found ByKerry Iles
Year Found2017
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates40.46256 N, 79.9319 W Map
Discovery NotesThis phage was isolated from the plant Trifolium repens, which was collected by Andrea Fetters. The plant was washed with PYCa, filtered, and then enriched with host at 30 degrees Celsius.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 6, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1102
Genome length (bp)46950
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGTAGGCAT
GC Content62.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCT
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesVariable morphology--mostly small plaques, but plaques become larger when fewer PFU are plates.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Margaret_1
(DNA binding protein)

Margaret_2
(methyltransferase)

Margaret_3

Margaret_4

Margaret_5

Margaret_6

Margaret_7
(terminase)

Margaret_8
(portal protein)

Margaret_9
(capsid maturation protease)

Margaret_10
(scaffolding protein)

Margaret_11
(major capsid protein)

Margaret_12
(head-to-tail connector complex protein)

Margaret_13
(head-to-tail connector complex protein)

Margaret_14
(head-to-tail connector complex protein)

Margaret_15
(head-to-tail connector complex protein)

Margaret_16
(major tail protein)

Margaret_17
(tail assembly chaperone)

Margaret_18
(tail assembly chaperone)

Margaret_19
(tape measure protein)

Margaret_20
(minor tail protein)

Margaret_21
(minor tail protein)

Margaret_22
(minor tail protein)

Margaret_23
(lysin A, protease M15 domain)

Margaret_24
(lysin A, glycosyl hydrolase domain)

Margaret_25
(holin)

Margaret_26
(membrane protein)

Margaret_27

Margaret_28

Margaret_29

Margaret_30

Margaret_31

Margaret_32

Margaret_33

Margaret_34

Margaret_35

Margaret_36

Margaret_37
(helix-turn-helix DNA binding domain protein)

Margaret_38

Margaret_39
(SprT-like protease)

Margaret_40

Margaret_41
(DNA binding protein)

Margaret_42
(helix-turn-helix DNA binding domain protein)

Margaret_43

Margaret_44
(nuclease)

Margaret_45

Margaret_46
(DNA recombinase)

Margaret_47

Margaret_48
(helicase)

Margaret_49
(DNA polymerase I)

Margaret_50
(deoxycytidylate deaminase)

Margaret_51
(lysin B)

Margaret_52
(thymidylate synthase)

Margaret_53

Margaret_54

Margaret_55

Margaret_56
(nucleotide pyrophosphohydrolase)

Margaret_57
(thymidylate kinase)

Margaret_58
(helicase)

Margaret_59
(DNA binding protein)

Margaret_60

Margaret_61

Margaret_62

Margaret_63
(DNA recombinase)

Margaret_64

Margaret_65

Margaret_66

Margaret_67

Margaret_68

Margaret_69

Margaret_70

Margaret_71

Margaret_72

Margaret_73

Margaret_74
(membrane protein)

Margaret_75
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH271302
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 7
Pitt Freezer Box Grid# F8
Available Files
Plaque PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload