The Actinobacteriophage Database | Phage MeganTheeKilla

Streptomyces phage MeganTheeKilla

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Detailed Information for Phage MeganTheeKilla
Discovery Information
Isolation Host	Streptomyces viridochromogenes DSM40736
Found By	Jennifer Olabode, Khushi Talati
Year Found	2019
Location Found	Richmond, TX United States
Finding Institution	Washington University in St. Louis
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	88°C
GPS Coordinates	29.666 N, 95.731 W Map
Discovery Notes	The soil is hard, dry, and crumbly. The depth of the hole is 6 cm. The soil was from a patch of dying grass in the backyard of Jennifer's house.
Naming Notes	MeganTheeStallion is an up-and-coming rapper from Houston. Because the sample was found in Houston, we dedicated our phage to this artist.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jun 15, 2020
Sequencing Facility	The McDonnell Genome Institute at Washington University
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	93
Genome length (bp)	128152
Character of genome ends	Direct Terminal Repeat
Direct Terminal Repeat Length	766 bp
GC Content	47.4%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	BK
Subcluster	BK1
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View Annadreamy (BK1) Belfort (BK1) Beuffert (BK1) Blueeyedbeauty (BK1) Comrade (BK1) Emma1919 (BK1) Faust (BK1) Forrest (BK1) Francob (BK1) Gilson (BK1) Jada (BK1) Karp (BK1) Kenrey (BK1) Limpid (BK1) MeganTheeKilla (BK1) Moab (BK1) Patelgo (BK1) Phredrick (BK1) SeresaTree (BK1) Sham (BK1) SparkleGoddess (BK1) Stigma (BK1) TunaTartare (BK1) BillNye (BK2) Circinus (BK2) Muntaha (BK2) Wakanda (BK2)
Annotating Institution	Washington University in St. Louis
Annotation Status	In GenBank
Plaque Notes	The plaques formed on our plates were consistently sized, often between 0.5 and 1.5 mm in diameter, with clean and distinct edges. Most plaques were circular in shape, if not perfectly round.
Morphotype	Siphoviridae
Has been Phamerated?	Yes
Gene List	Click to View MeganTheeKilla_1 MeganTheeKilla_2 MeganTheeKilla_3 MeganTheeKilla_4 MeganTheeKilla_5 MeganTheeKilla_6 MeganTheeKilla_7 (DNA methyltransferase) MeganTheeKilla_8 (ThyX-like thymidylate synthase) MeganTheeKilla_9 MeganTheeKilla_10 MeganTheeKilla_11 MeganTheeKilla_12 MeganTheeKilla_13 MeganTheeKilla_18 MeganTheeKilla_19 (hydrolase) MeganTheeKilla_20 (glycosyltransferase) MeganTheeKilla_21 (glycosyltransferase) MeganTheeKilla_22 (ribonucleotide reductase) MeganTheeKilla_23 (endolysin) MeganTheeKilla_24 MeganTheeKilla_25 MeganTheeKilla_26 (portal protein) MeganTheeKilla_27 MeganTheeKilla_28 (capsid maturation protease) MeganTheeKilla_29 (major capsid protein) MeganTheeKilla_30 MeganTheeKilla_31 (head-to-tail adaptor) MeganTheeKilla_32 (head-to-tail stopper) MeganTheeKilla_33 MeganTheeKilla_34 MeganTheeKilla_35 (major tail protein) MeganTheeKilla_36 (tail assembly chaperone) MeganTheeKilla_37 (tail assembly chaperone) MeganTheeKilla_38 (tape measure protein) MeganTheeKilla_39 (minor tail protein) MeganTheeKilla_40 (minor tail protein) MeganTheeKilla_41 MeganTheeKilla_42 (minor tail protein) MeganTheeKilla_43 (minor tail protein) MeganTheeKilla_44 MeganTheeKilla_45 MeganTheeKilla_46 MeganTheeKilla_47 (HNH endonuclease) MeganTheeKilla_48 (metallophosphoesterase) MeganTheeKilla_49 MeganTheeKilla_50 (MerR-like helix-turn-helix DNA binding domain protein) MeganTheeKilla_51 MeganTheeKilla_52 MeganTheeKilla_53 MeganTheeKilla_54 (DnaB-like dsDNA helicase) MeganTheeKilla_55 MeganTheeKilla_56 (DNA primase) MeganTheeKilla_57 (ssDNA binding protein) MeganTheeKilla_58 (HNH endonuclease) MeganTheeKilla_59 (DnaE-like DNA polymerase III alpha) MeganTheeKilla_60 MeganTheeKilla_61 (MazG-like nucleotide pyrophosphohydrolase) MeganTheeKilla_62 MeganTheeKilla_63 (RecA-like DNA recombinase) MeganTheeKilla_64 MeganTheeKilla_65 MeganTheeKilla_66 (PnuC-like nicotinamide riboside transporter) MeganTheeKilla_67 (Holliday junction resolvase) MeganTheeKilla_68 MeganTheeKilla_69 MeganTheeKilla_70 (Cas4 family exonuclease) MeganTheeKilla_71 MeganTheeKilla_72 (RuvC-like resolvase) MeganTheeKilla_73 MeganTheeKilla_74 (DprA-like ssDNA binding protein) MeganTheeKilla_75 (helix-turn-helix DNA-binding domain protein) MeganTheeKilla_76 (glycosyltransferase) MeganTheeKilla_77 (glycosyltransferase) MeganTheeKilla_78 (nucleotidyltransferase) MeganTheeKilla_79 MeganTheeKilla_80 (DnaQ-like DNA polymerase III subunit) MeganTheeKilla_81 (deoxynucleoside monophosphate kinase) MeganTheeKilla_82 (5' nucleotidase) MeganTheeKilla_83 MeganTheeKilla_84 MeganTheeKilla_85 MeganTheeKilla_86 MeganTheeKilla_87 (terminase large subunit) MeganTheeKilla_88 MeganTheeKilla_89 MeganTheeKilla_92 (HNH endonuclease) MeganTheeKilla_93 MeganTheeKilla_96 MeganTheeKilla_99 MeganTheeKilla_101 MeganTheeKilla_102 MeganTheeKilla_103 MeganTheeKilla_104 (Ro-like RNA binding protein) MeganTheeKilla_105 MeganTheeKilla_106 MeganTheeKilla_107 MeganTheeKilla_108 MeganTheeKilla_110 MeganTheeKilla_111 (DnaQ-like DNA polymerase III subunit) MeganTheeKilla_112 MeganTheeKilla_113 MeganTheeKilla_115 MeganTheeKilla_117 MeganTheeKilla_121 MeganTheeKilla_122 MeganTheeKilla_123 MeganTheeKilla_124 MeganTheeKilla_130 MeganTheeKilla_131 MeganTheeKilla_133 MeganTheeKilla_135 MeganTheeKilla_136 (band-7-like membrane protein) MeganTheeKilla_137 MeganTheeKilla_138 MeganTheeKilla_139 MeganTheeKilla_140 MeganTheeKilla_142 MeganTheeKilla_143 MeganTheeKilla_144 MeganTheeKilla_146 MeganTheeKilla_147 (RNA ligase) MeganTheeKilla_148 MeganTheeKilla_149 MeganTheeKilla_150 (polynucleotide kinase) MeganTheeKilla_151 MeganTheeKilla_152 MeganTheeKilla_153 (HNH endonuclease) MeganTheeKilla_155 MeganTheeKilla_156 MeganTheeKilla_157 MeganTheeKilla_158 MeganTheeKilla_161 MeganTheeKilla_162 MeganTheeKilla_163 MeganTheeKilla_164 MeganTheeKilla_165 MeganTheeKilla_166 MeganTheeKilla_167 MeganTheeKilla_168 MeganTheeKilla_169 MeganTheeKilla_170 (rIIB-like protein) MeganTheeKilla_171 MeganTheeKilla_172 (metallophosphoesterase) MeganTheeKilla_173 MeganTheeKilla_174 MeganTheeKilla_176 MeganTheeKilla_177 (WhiB family transcription factor) MeganTheeKilla_178 MeganTheeKilla_179 MeganTheeKilla_180 MeganTheeKilla_182 MeganTheeKilla_183 MeganTheeKilla_184 MeganTheeKilla_187 MeganTheeKilla_189 MeganTheeKilla_191 MeganTheeKilla_193 MeganTheeKilla_194 MeganTheeKilla_195 MeganTheeKilla_196 MeganTheeKilla_197 MeganTheeKilla_198 MeganTheeKilla_199 MeganTheeKilla_200 MeganTheeKilla_201 (thioredoxin) MeganTheeKilla_202 MeganTheeKilla_203 MeganTheeKilla_204 MeganTheeKilla_205 MeganTheeKilla_206 MeganTheeKilla_207 MeganTheeKilla_208 MeganTheeKilla_209 MeganTheeKilla_210 (RNA ligase) MeganTheeKilla_211 MeganTheeKilla_212 MeganTheeKilla_213 MeganTheeKilla_214 MeganTheeKilla_215 MeganTheeKilla_216 MeganTheeKilla_217 MeganTheeKilla_218 MeganTheeKilla_219 MeganTheeKilla_220 MeganTheeKilla_221 (Lsr2-like DNA bridging protein) MeganTheeKilla_222 MeganTheeKilla_223 MeganTheeKilla_224 MeganTheeKilla_225 MeganTheeKilla_226 MeganTheeKilla_227 MeganTheeKilla_228 MeganTheeKilla_229 (HNH endonuclease) MeganTheeKilla_230 MeganTheeKilla_231 MeganTheeKilla_232 MeganTheeKilla_233 (peptidase) MeganTheeKilla_234 MeganTheeKilla_235 MeganTheeKilla_236 MeganTheeKilla_237 MeganTheeKilla_238 MeganTheeKilla_239 MeganTheeKilla_240 MeganTheeKilla_241 (metallophosphoesterase) MeganTheeKilla_242 MeganTheeKilla_243 (SprT-like protease) MeganTheeKilla_244 MeganTheeKilla_245 MeganTheeKilla_246 MeganTheeKilla_247 MeganTheeKilla_248 MeganTheeKilla_249 MeganTheeKilla_251 MeganTheeKilla_252 MeganTheeKilla_253 (kinase) MeganTheeKilla_254 MeganTheeKilla_255 MeganTheeKilla_256 MeganTheeKilla_257 MeganTheeKilla_258 MeganTheeKilla_259 MeganTheeKilla_260 MeganTheeKilla_261 MeganTheeKilla_262 MeganTheeKilla_263 MeganTheeKilla_264 MeganTheeKilla_265 MeganTheeKilla_266 MeganTheeKilla_267 MeganTheeKilla_268 MeganTheeKilla_269 MeganTheeKilla_270
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MW435853
Refseq Number	None yet
Archiving Info
Archiving status	Not in Pitt Archives
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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