Mycobacterium phage PepperEW
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| Detailed Information for Phage PepperEW | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Andra Ionescu |
| Year Found | 2012 |
| Location Found | La Jolla, CA USA |
| Finding Institution | University of California, San Diego |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 32.52586 N, 117.14014 W Map |
| Discovery Notes | discovered on 10/2/2012 sample taken from Earl Warren Community Garden in Earl Warren College at UC San Diego taken from loose potting soil containing organic matter, slightly moist, dry at the soil's surface depth: ~30 cm below soil surface temperature:~80 degrees fahrenheit |
| Naming Notes | Pepper: named for the minute size of the plaque the bacteriophage produced (less than 1 mm in diameter) as well as the morphological appearance of these plaques EW: location where phage was found (Earl Warren College) |
| Sequencing Information | |
| Sequencing Complete? | No |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Size: slightly <1 mm in diameter Appearance: majority of plaques were clear; some plaques were turbid in the earlier rounds of purification. All plaques were clear during later rounds of purification, indicating that the phage was temperate and underwent induction, switching to a lytic lfestyle over time Incubation Time: 24 hours (minimum incubation time) Incubation Temperature: 37 degrees Celsius |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 2.2 x 10 ^ 10 pfu/mL |
| Date of SEA Lysate Titering | Nov 2, 2012 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |