Microbacterium phage Piroshky
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| Detailed Information for Phage Piroshky | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Lucia Tilson |
| Year Found | 2023 |
| Location Found | Pullman, WA United States |
| Finding Institution | Washington State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 46.733 N, 117.168 W Map |
| Discovery Notes | Piroshky was isolated from a soil sample that was gathered from under a dumpster near the Pi Beta Phi sorority house on the WSU Pullman campus. The GPS coordinates for the collection location of our phage were (46.733, -117.168). The sample was taken 2 to 3cm below the surface and contained high moisture, pieces of decaying leaves, litter, and road gravel from the nearby parking lot. The soil was gathered on Tuesday, February 28th, 2023, which was in week 7 of the Biology 107 labs this semester. |
| Naming Notes | This phage is of the Microbacterium foliorum strain, which is really awesome and mind-boggling to me. We decided to name it Piroshky because it is just as cool and unique as the piroshkis from the Seattle Pike Place Market. I love the Pike Place Market piroshkis, and I now love our bacteriophage Piroshky. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | On our plaque assays, there were many phage plaques that were easy to identify and streak out. In the middle of one of the plaque assays, there was also a cluster of antibiotics that were repelling other bacteria and bits of contamination growth in the agar. There were also many phages on our serial dilution plates. On our sixth dilution, there were 26 plaques counted! Other plates also showed interesting and unique results, but we chose to use the sixth diluted plate for further study. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |