PhagesDB Blog Posts Tagged: Sequencing
Dan Russell
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Feb 1, 2024

What is DOGEMS?


Deconvolution Of Genomes after En Masse Sequencing, or DOGEMS (DAH-jums), is an approach to sequencing phages where individually isolated phage DNA samples are deliberately mixed before preparing libraries. The main reasons to do so are to reduce library …

Dan Russell
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Jan 23, 2018

Gene Content Similarity and Dissimilarity


Last year, one of the grad students in the Hatfull lab, Travis Mavrich, was the lead author on a paper in Nature Microbiology that explored the extent to which horizontal gene transfer and mosaicism …

Dan Russell
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Jan 26, 2015

I've gotten a few questions recently about doing phage genome sequencing on Illumina sequencers, and we ourselves have been pumping genomes through our Illumina MiSeq and loving it. But I wanted to issue a word of caution to anyone considering …

Tags: Sequencing
Dan Russell
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Oct 31, 2014
Recently, Eric Miller at NC State asked me about phage genome ends, and how a 3' overhang (sticky end) would look in an assembled genome compared to how a 5' overhang would look. So I made a picture! Hopefully it …
Dan Russell
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Nov 25, 2013
Many of you in the SEA have been doing some phage sequencing on your own, and some of you have been using Ion Torrent PGMs to do that sequencing. We at Pitt have been working with our PGM for over …
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