| Detailed Information for Phage AllBusiness |
| Discovery Information |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | James Grout and Anastasiya Pikulik |
| Year Found | 2024 |
| Location Found | Spokane, WA USA |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 21°C |
| GPS Coordinates | 47.66639 N, 117.40457 W Map |
| Naming Notes | My partner and I were not interested in coming up with a name for the phage. Our instructor and TA suggested the name AllBusiness. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 13, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 5505 |
| Genome length (bp) | 43416 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | TCCGCCGCGTAA |
| GC Content | 65.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | FF |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Gonzaga University |
| Annotation Status | QC Complete |
| Plaque Notes | We were looking for a plate that was not crowded and could adequately portray our phage. Based on our phage morphology photo, we could see that our phage seems symmetrical and perfectly circular. The average size of our plaques is 3.2mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 64 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
192 |
| Pitt Freezer Box Grid# |
A1 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
