| Detailed Information for Phage Azrael100 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Edith Machowski |
| Year Found | 2021 |
| Location Found | Johannesburg, South Africa |
| Finding Institution | University of the Witwatersrand |
| Program | Unavailable |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 26.188846 S, 28.00529 E Map |
| Discovery Notes | The soil sample was taken from a warm compost heap in the sun. |
| Naming Notes | Our previous phage was named after a pet dog (Lopsy). Our pet cat names were already on the data base (Spots and STripes). Azrael is the pet cat of Gargamel who wants to destroy the Smurfs. There 100 Smurfs. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 26, 2023 |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 309 |
| Genome length (bp) | 78063 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | ACCACTGCAAC |
| GC Content | 56.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | V |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | V cluster phages are lytic. There is no attP site or integrase gene. |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are very small (~1mm) and clear with no halo. |
| Morphotype | Siphoviridae |
| Number of Genes | 141 |
| Number of tRNAs | 21 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR199846 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
