Gordonia phage Basils
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| Detailed Information for Phage Basils | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Katharine Kleinbrahm |
| Year Found | 2019 |
| Location Found | Pittsburgh, PA United States |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.4423 N, 79.9575 W Map |
| Discovery Notes | -Collected from the corner of De Soto Street and 5th Ave -3800 Fifth Ave (Google Maps formulated address) - Collected on Monday, September 2nd, 2019 at 6:35 pm -Observations: - the soil was very moist, most probably due to the rainfall that had occurred the night before -It was partly cloudy outside and the temperature was 75 degrees Fahrenheit -There is a tree located about 3 feet from the site of the collection -the soil was partially covered in mulch; however, before collection, some mulch was brushed away to allow a more homogenous collection of soil -the soil was surrounded by concrete, and there were a few rocks scattered throughout the soil collection spot -The sample was stored for about 21 hours inside of a plastic bag. The plastic bag was kept inside of a room temperature drawer during this time. It took approximately 5 minutes for the soil sample to be transported from the location spot to the place of storage. |
| Naming Notes | My high school, Saint Basil Academy, has the nickname Basils. I credit my desire to learn and succeed to the skills and knowledge I learned there and the empowerment I gained during my time there. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | This phage contains two different morphologies. This conclusion was made after many rounds of purification. Both morphologies of this plaque are characterized by clear, circular plaques. However, some of the plaques are small (1.5mm), while others are medium in size (~2.5mm) |
| Morphotype | Podoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 4.9*10^8 pfu/ml |
| Pitt Freezer Box# | 113 |
| Pitt Freezer Box Grid# | F12 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |