Microbacterium phage Big4
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Detailed Information for Phage Big4
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Former namesB1g4
Found ByYash Patel
Year Found2021
Location Foundbloomington, IL United States
Finding InstitutionIllinois Wesleyan University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates40.490278 N, 88.992222 W Map
Discovery NotesWhen I plated my lysate with other host it was contaminate.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 13, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage384
Genome length (bp)192363
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length17669 bp
GC Content58.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterGD
SubclusterGD2
Cluster Life CycleUnknown
Lysogeny Notesit wasn't able to isolate a lysogen
Other Cluster Members
Annotating InstitutionIllinois Wesleyan University
Annotation StatusIn GenBank
Plaque NotesIt had small plaque, but It had multiple flooded plates with plaques.
Number of Genes318
Number of tRNAs48
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Big4_1

Big4_2

Big4_3

Big4_4
(endolysin)

Big4_5

Big4_6

Big4_7

Big4_8

Big4_9

Big4_10

Big4_11

Big4_12

Big4_13

Big4_14
(SprT-like protease)

Big4_15

Big4_16

Big4_17

Big4_18

Big4_19

Big4_20
(NrdH-like glutaredoxin)

Big4_21

Big4_22

Big4_23

Big4_24

Big4_25

Big4_26

Big4_27

Big4_28

Big4_29

Big4_30

Big4_31

Big4_32

Big4_33

Big4_34

Big4_35

Big4_36

Big4_37

Big4_38

Big4_39

Big4_40

Big4_41

Big4_42

Big4_43

Big4_44

Big4_45

Big4_46

Big4_47

Big4_48

Big4_49

Big4_50

Big4_51

Big4_52

Big4_53

Big4_54

Big4_55

Big4_56

Big4_57

Big4_58

Big4_59

Big4_60

Big4_61

Big4_62

Big4_63

Big4_64
(terminase)

Big4_65
(DNA binding protein)

Big4_66

Big4_67

Big4_68
(terminase large subunit)

Big4_69
(terminase large subunit)

Big4_70

Big4_71
(portal protein)

Big4_72

Big4_73

Big4_74

Big4_75

Big4_76

Big4_77

Big4_78

Big4_79
(major tail protein)

Big4_80
(head-to-tail adaptor)

Big4_81

Big4_82

Big4_83

Big4_84
(tail assembly chaperone)

Big4_85

Big4_86
(tape measure protein)

Big4_87
(minor tail protein)

Big4_88
(minor tail protein)

Big4_89
(minor tail protein)

Big4_90

Big4_91

Big4_92

Big4_93
(DNA primase/helicase)

Big4_94

Big4_95

Big4_96
(DnaQ-like DNA polymerase III subunit)

Big4_97
(WhiB family transcription factor)

Big4_98

Big4_99

Big4_100

Big4_101
(RecA-like DNA recombinase)

Big4_102
(Cas4 family exonuclease)

Big4_103
(DNA helicase)

Big4_104
(RuvC-like resolvase)

Big4_105
(DNA polymerase I)

Big4_106

Big4_107 — tRNA
(tRNA-Pro(tgg))

Big4_108 — tRNA
(tRNA-Pro(cgg))

Big4_109 — tRNA
(tRNA-Pro(ggg))

Big4_110 — tRNA
(tRNA-His(gtg))

Big4_111 — tRNA
(tRNA-Met(cat))

Big4_112 — tRNA
(tRNA-Asp(gtc))

Big4_113

Big4_114 — tRNA
(tRNA-Asp(gtc))

Big4_115 — tRNA
(tRNA-Glu(ctc))

Big4_116 — tRNA
(tRNA-Glu(ttc))

Big4_118 — tRNA
(tRNA-Tyr(gta))

Big4_119

Big4_120 — tRNA
(tRNA-Phe(gaa))

Big4_121 — tRNA
(tRNA-Phe(gaa))

Big4_122 — tRNA
(tRNA-Leu(cag))

Big4_123 — tRNA
(tRNA-Leu(tag))

Big4_124 — tRNA
(tRNA-Leu(caa))

Big4_125 — tRNA
(tRNA-Leu(gag))

Big4_126

Big4_127
(peptidyl tRNA hydrolase)

Big4_128

Big4_129
(MazG-like nucleotide pyrophosphohydrolase)

Big4_130
(ThyX-like thymidylate synthase)

Big4_131

Big4_132 — tRNA
(tRNA-Ser(gga))

Big4_133

Big4_134

Big4_135 — tRNA
(tRNA-Ser(gct))

Big4_136 — tRNA
(tRNA-Lys(ctt))

Big4_137 — tRNA
(tRNA-Lys(ctt))

Big4_138 — tRNA
(tRNA-Gly(tcc))

Big4_139 — tRNA
(tRNA-Gly(gcc))

Big4_140 — tRNA
(tRNA-Gly(gcc))

Big4_141 — tRNA
(tRNA-Thr(tgt))

Big4_142 — tRNA
(tRNA-Thr(cgt))

Big4_143 — tRNA
(tRNA-Thr(ggt))

Big4_144 — tRNA
(tRNA-Val(gac))

Big4_145

Big4_146 — tRNA
(tRNA-Val(tac))

Big4_147
(HNH endonuclease)

Big4_148 — tRNA
(tRNA-Asn(gtt))

Big4_149 — tRNA
(tRNA-Asn(gtt))

Big4_150 — tRNA
(tRNA-Trp(cca))

Big4_151 — tRNA
(tRNA-Trp(cca))

Big4_152 — tRNA
(tRNA-Arg(acg))

Big4_153 — tRNA
(tRNA-Arg(acg))

Big4_154 — tRNA
(tRNA-Arg(ccg))

Big4_155 — tRNA
(tRNA-Arg(cct))

Big4_156

Big4_157 — tRNA
(tRNA-Gln(ctg))

Big4_158 — tRNA
(tRNA-Gln(ctg))

Big4_159 — tRNA
(tRNA-Gln(ttg))

Big4_160 — tRNA
(tRNA-Lys(ttt))

Big4_161

Big4_162

Big4_163
(ADP-ribosyltransferase)

Big4_164

Big4_165
(RtcB-like RNA ligase)

Big4_166

Big4_167

Big4_168

Big4_169

Big4_170 — tRNA
(tRNA-Met(cat))

Big4_171 — tRNA
(tRNA-Met(cat))

Big4_172 — tRNA
(tRNA-Cys(gca))

Big4_173

Big4_174

Big4_175

Big4_176 — tRNA
(tRNA-Ile(gat))

Big4_178 — tRNA
(tRNA-Ala(tgc))

Big4_179 — tRNA
(tRNA-Ala(tgc))

Big4_180 — tRNA
(tRNA-Ala(ggc))

Big4_182 — tRNA
(tRNA-Ser(cga))

Big4_183

Big4_184
(endolysin)

Big4_185
(Ro-like RNA binding protein)

Big4_186

Big4_187

Big4_188

Big4_189

Big4_190
(DNA helicase)

Big4_191

Big4_192

Big4_193

Big4_194

Big4_195

Big4_196

Big4_197

Big4_198

Big4_199

Big4_200
(ribonucleotide reductase)

Big4_201

Big4_202
(ribonucleotide reductase)

Big4_203
(NrdI-like flavodoxin)

Big4_204
(glutaredoxin)

Big4_205

Big4_206

Big4_207

Big4_208

Big4_209

Big4_210
(nucleotidyltransferase)

Big4_211

Big4_212
(phosphoesterase)

Big4_213
(metallophosphoesterase)

Big4_214
(phosphatase)

Big4_215

Big4_216

Big4_217

Big4_218

Big4_219
(phosphatase)

Big4_220

Big4_221

Big4_222
(deoxynucleoside monophosphate kinase)

Big4_223

Big4_224

Big4_225
(AAA-ATPase)

Big4_226
(rIIA-like protein)

Big4_227
(rIIB-like protein)

Big4_228

Big4_229

Big4_230

Big4_231

Big4_232
(phosphoribosyl transferase)

Big4_233

Big4_234

Big4_235

Big4_236

Big4_237

Big4_238
(nicotinate ribosyltransferase)

Big4_239

Big4_240

Big4_241

Big4_242

Big4_243

Big4_244

Big4_245

Big4_246
(PnuC-like nicotinamide riboside transporter)

Big4_247

Big4_248

Big4_249

Big4_250
(adenylyltransferase)

Big4_251

Big4_252

Big4_253
(SpoT-like ppGpp synthesis/degradation protein)

Big4_254

Big4_255
(tRNA nucleotidyltransferase)

Big4_256

Big4_257

Big4_258

Big4_259

Big4_260

Big4_261

Big4_262

Big4_263
(exonuclease)

Big4_264

Big4_265

Big4_266

Big4_267

Big4_268

Big4_269

Big4_270
(exonuclease)

Big4_271

Big4_272

Big4_273

Big4_274

Big4_275

Big4_276

Big4_277

Big4_278

Big4_279

Big4_280

Big4_281

Big4_282

Big4_283

Big4_284

Big4_285

Big4_286

Big4_287

Big4_288

Big4_289

Big4_290

Big4_291

Big4_292

Big4_293

Big4_294

Big4_295

Big4_296

Big4_297

Big4_298

Big4_299

Big4_300

Big4_301

Big4_302

Big4_303

Big4_304

Big4_305

Big4_306

Big4_307

Big4_308

Big4_309

Big4_310

Big4_311

Big4_312

Big4_313

Big4_314

Big4_315

Big4_316

Big4_317
(AAA-ATPase)

Big4_318

Big4_319

Big4_320

Big4_321

Big4_322
(AAA-ATPase)

Big4_323

Big4_324

Big4_325

Big4_326

Big4_327

Big4_328

Big4_329

Big4_330
(endolysin)

Big4_331

Big4_332

Big4_333

Big4_334

Big4_335

Big4_336

Big4_337

Big4_338

Big4_339

Big4_340
(SprT-like protease)

Big4_341

Big4_342

Big4_343

Big4_344

Big4_345

Big4_346
(NrdH-like glutaredoxin)

Big4_347

Big4_348

Big4_349

Big4_350

Big4_351

Big4_352

Big4_353

Big4_354

Big4_355

Big4_356

Big4_357

Big4_358

Big4_359

Big4_360

Big4_361

Big4_362

Big4_363

Big4_364

Big4_365

Big4_366

Big4_367

Big4_368

Big4_369

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON637770
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.6x10^10
Pitt Freezer Box# 142
Pitt Freezer Box Grid# E4
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload