Mycobacterium phage Buffy
Buffy Plaque Picture
Buffy EM Picture
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Detailed Information for Phage Buffy
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found BySydney Schneider
Year Found2012
Location FoundPullman, WA USA
Finding InstitutionWashington State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates46.731689 N, 117.167603 W Map
Discovery NotesDiscovered 8/30/12 behind Honors Hall dorm on a slope with bushes and vegetation that is regularly watered. Soil gathered from about 4cm deem at a temperature of 69 degrees Fahrenheit.
Naming NotesNamed after Buffy Ann Summers played by Sarah Michelle Gellar on the most amazing show ever: Buffy the Vampire Slayer. She kills vampires and her phage kills bacteria.
Sequencing Information
Sequencing Complete?No
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesAlmost perfectly round plaques ranging from 1 mm to 5 mm. They appear very clear at lower concentrations, but very cloudy at higher concentrations after having lysed the entire bacterial lawn on a plate.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2012WASUbuffySLS
SEA Lysate Titer 8.2 x 10^9 pfu/microliter
Date of SEA Lysate Titering Nov 8, 2012
Pitt Freezer Box# 42
Pitt Freezer Box Grid# 37
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload