Mycobacterium phage Bulletproof
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| Detailed Information for Phage Bulletproof | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Anthony Brusnahan |
| Year Found | 2013 |
| Location Found | Columbus, OH USA |
| Finding Institution | The Ohio State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.001674 N, 83.013416 W Map |
| Discovery Notes | Found at the surface of mud-like, chunky soil. The site is a high traffic area was under construction during this period. Date: 9-13-13. Time: 4:20pm. Temperature: 68 degrees Fahrenheit. |
| Naming Notes | The name of the phage came from the difficulty of producing a plate from which the HTL titer could be determined. The 10^-24 solution yielded a calculable titer. It seemed impossible to put a dent into the number of plaques that the phage produced; thus giving it the appropriate name of Bulletproof. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques originally had a bullseye formation, but as the concentration of phage increased, the size of the plaques decreased; thus hindering the visibility and formation of a bullseye. The plaques were around 5 mm in diameter before purification. After purification, the plaques were roughly 1.0-1.5 mm in diameter. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.56X10^29 |
| Pitt Freezer Box# | 3 |
| Pitt Freezer Box Grid# | D6 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
