Mycobacterium phage Candle
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Detailed Information for Phage Candle
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByEmma Ryan
Year Found2018
Location FoundNorthport, AL USA
Finding InstitutionUniversity of West Alabama
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates33.259722 N, 87.610556 W Map
Discovery NotesI found this phage's soil sample at the base of an okra stalk. The soil was mostly topsoil, recently tilled for planting the okra and very dry. Outside, it was very hot. I recorded 87 degrees Fahrenheit.
Naming NotesUpon isolating the phage, I noticed that there were halos surrounding each individual plaque. These halos resemble the light orb that surround a lit candle in a dark room.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 28, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage564
Genome length (bp)71390
Character of genome endsCircularly Permuted
GC Content56.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterR
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of West Alabama
Annotation StatusIn GenBank
Plaque NotesThe diluted plaques are very small, pinpricked-size. There are small halos surrounding each individual plaque, extending a few millimeters out from the center.
MorphotypeSiphoviridae
Number of Genes95
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Candle_1

Candle_2

Candle_3

Candle_4
(terminase)

Candle_5

Candle_6
(portal protein)

Candle_7

Candle_8

Candle_9

Candle_10

Candle_11

Candle_12
(MuF-like minor capsid protein)

Candle_13

Candle_14

Candle_15

Candle_16
(major capsid protein)

Candle_17

Candle_18

Candle_19

Candle_20

Candle_21

Candle_22

Candle_23

Candle_24
(major tail protein)

Candle_25
(tail assembly chaperone)

Candle_26
(tail assembly chaperone)

Candle_27
(tape measure protein)

Candle_28
(minor tail protein)

Candle_29
(minor tail protein)

Candle_30
(minor tail protein)

Candle_31
(minor tail protein)

Candle_32

Candle_33

Candle_34

Candle_35
(lysin A)

Candle_36

Candle_37

Candle_38

Candle_39
(lysin B)

Candle_40
(NrdH-like glutaredoxin)

Candle_41

Candle_42

Candle_43

Candle_44

Candle_45

Candle_46

Candle_47

Candle_48

Candle_49

Candle_50

Candle_51

Candle_52

Candle_53

Candle_54

Candle_55

Candle_56

Candle_57

Candle_58

Candle_59

Candle_60

Candle_61
(O-methyltransferase)

Candle_62

Candle_63

Candle_64
(DNA helicase)

Candle_65

Candle_66

Candle_67
(Cas4 family exonuclease)

Candle_68
(RecA-like DNA recombinase)

Candle_69

Candle_70
(DNA polymerase III sliding clamp)

Candle_71

Candle_72
(DnaE-like DNA polymerase III)

Candle_73

Candle_74
(thimidylate synthase)

Candle_75

Candle_76
(band-7-like membrane protein)

Candle_77

Candle_78

Candle_79

Candle_80

Candle_81
(RuvC-like resolvase)

Candle_82

Candle_83

Candle_84

Candle_85
(MazG-like nucleotide pyrophosphohydrolase)

Candle_86

Candle_87
(DNA primase/polymerase)

Candle_88

Candle_89
(methyltransferase)

Candle_90

Candle_91

Candle_92

Candle_93

Candle_94

Candle_95

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK757446
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 85
Pitt Freezer Box Grid# E9
Available Files
GenBank File for PhameratorDownload