| Detailed Information for Phage Colossa | |
| Discovery Information | |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Brandon Mitchell Hernandez |
| Year Found | 2024 |
| Location Found | Durham, NC United States |
| Finding Institution | Durham Technical Community College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 25°C |
| GPS Coordinates | 36.025984 N, 78.785948 W Map |
| Discovery Notes | I discovered my phage in the backyard soil of my mother’s house, where there are chickens that frequently burrow and a vegetable garden. The chickens’ activity regularly disturbs the soil, adding organic matter that enriches the environment. The soil’s close proximity to vegetable plants and natural fertilizer from the chickens creates a nutrient-rich habitat. This setting likely supports a diverse microbial community influenced by both animal and plant life, offering an ideal environment for unique phage discovery. |
| Naming Notes | The name Colossa is inspired by the Colossal Titan from Attack on Titan, symbolizing a large or powerful phage. The choice of "Colossa" maintains a professional tone while reflecting the strength and impact often associated with the Colossal Titan. This name is easily pronounceable, adheres to SEA-PHAGES guidelines, and avoids any complex capitalization or systematic numbering. It’s a fitting name for a phage discovered in a rich, nutrient-dense environment where significant microbial diversity might be expected. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jun 16, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 310 |
| Genome length (bp) | 84865 |
| Character of genome ends | Circularly Permuted |
| GC Content | 67.7% |
| Sequencing Notes | This phage was sequenced as part of a DOGEMS sample. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | KA |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members | |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Planless |
| Number of Genes | 167 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewColossa_1 Colossa_2 Colossa_3 Colossa_4 Colossa_5 Colossa_6 Colossa_7 Colossa_8 Colossa_9 Colossa_10 Colossa_11 Colossa_12 Colossa_13 Colossa_14 Colossa_15 Colossa_16 Colossa_17 Colossa_18 Colossa_19 Colossa_20 Colossa_21 Colossa_22 Colossa_23 Colossa_24 Colossa_25 Colossa_26 Colossa_27 Colossa_28 Colossa_29 Colossa_30 Colossa_31 Colossa_32 Colossa_33 Colossa_34 Colossa_35 Colossa_36 Colossa_37 Colossa_38 Colossa_39 Colossa_40 Colossa_41 Colossa_42 Colossa_43 Colossa_44 Colossa_45 Colossa_46 Colossa_47 Colossa_48 Colossa_49 Colossa_50 Colossa_51 Colossa_52 Colossa_53 Colossa_54 Colossa_55 Colossa_56 Colossa_57 Colossa_58 Colossa_59 Colossa_60 Colossa_61 Colossa_62 Colossa_63 Colossa_64 Colossa_65 Colossa_66 Colossa_67 Colossa_68 Colossa_69 Colossa_70 Colossa_71 Colossa_72 Colossa_73 Colossa_74 Colossa_75 Colossa_76 Colossa_77 Colossa_78 Colossa_79 Colossa_80 Colossa_81 Colossa_82 Colossa_83 Colossa_84 Colossa_85 Colossa_86 Colossa_87 Colossa_88 Colossa_89 Colossa_90 Colossa_91 Colossa_92 Colossa_93 Colossa_94 Colossa_95 Colossa_96 Colossa_97 Colossa_98 Colossa_99 Colossa_100 Colossa_101 Colossa_102 Colossa_103 Colossa_104 Colossa_105 Colossa_106 Colossa_107 Colossa_108 Colossa_109 Colossa_110 Colossa_111 Colossa_112 Colossa_113 Colossa_114 Colossa_115 Colossa_116 Colossa_117 Colossa_118 Colossa_119 Colossa_120 Colossa_121 Colossa_122 Colossa_123 Colossa_124 Colossa_125 Colossa_126 Colossa_127 Colossa_128 Colossa_129 Colossa_130 Colossa_131 Colossa_132 Colossa_133 Colossa_134 Colossa_135 Colossa_136 Colossa_137 Colossa_138 Colossa_139 Colossa_140 Colossa_141 Colossa_142 Colossa_143 Colossa_144 Colossa_145 Colossa_146 Colossa_147 Colossa_148 Colossa_149 Colossa_150 Colossa_151 Colossa_152 Colossa_153 Colossa_154 Colossa_155 Colossa_156 Colossa_157 Colossa_158 Colossa_159 Colossa_160 Colossa_161 Colossa_162 Colossa_163 — tRNA Colossa_164 Colossa_165 Colossa_166 Colossa_167 Colossa_168 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 197 |
| Pitt Freezer Box Grid# | C9 |