| Detailed Information for Phage Cucurbita |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Amelia Sterenberg |
| Year Found | 2015 |
| Location Found | Grand Rapids, MI USA |
| Finding Institution | Calvin University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.933889 N, 85.591111 W Map |
| Discovery Notes | Sample taken from the Calvin Community Garden, from the soil underneath a squash plant, among decomposing plant material. Soil was moist. |
| Naming Notes | Cucurbita is the plant genus which includes squash plants.
|
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 9, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 971 |
| Genome length (bp) | 93686 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGCGACGCTC |
| GC Content | 62.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CQ |
| Subcluster | CQ1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques were at times variable in size, but picking larger or small plaques always led to variable sizes. The edges were very jagged and not extremely circular. They did not have halos and were clear. Toward the last two purifications and for the MTL and HTL production and titer, the plaques did become more uniform. |
| Morphotype | Siphoviridae |
| Number of Genes | 178 |
| Number of tRNAs | 8 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX557276 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
tbd |
| Pitt Freezer Box# |
26 |
| Pitt Freezer Box Grid# |
B12 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
