Arthrobacter phage Cuervo
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Detailed Information for Phage Cuervo
Discovery Information
Isolation HostArthrobacter globiformis B-2979
Found ByLucia Soto Herrera
Year Found2019
Location FoundChandler, AZ United States
Finding InstitutionArizona State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature37°C
GPS Coordinates33.420621 N, 111.932855 W Map
Discovery NotesThis phage was discovered in the soil of a garden pot that had a plant growing out of it. It was partially in the shade but received a decent amount of sunlight. The soil looked to be slightly moist. The garden pot was in a small garden that birds and stray cats are known to frequent so it is unknown albeit possible for there to be animal contact to the soil. The phage was thought to infect A. globiformis but it was discovered that the bacteria that the class had been using all along was actually a contaminant that had been isolated from the very first streak plate of the HHMI stock supply provided.

FOR GEL OF ENZYME DIGESTS: (left to right) ladder, uncut, NspI, SacI, BamHI,ClaI

NspI is the only one that cut the DNA
Naming NotesThe Spanish word for Crow since crow was already taken. I am absolutely fascinated by corvid studies and how intelligent these birds are. They can easily be trained to accomplish goals or understand human machines/patterns and I feel like that's part of what we do with phage.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Lysogeny NotesBased on plaques appears to be in lytic phase
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesThe plaques are small (about the size of a pinprick) and appear clear, however given time to incubate they expand to be about the size of a #2 pencil's eraser. The larger plaques do appear to have a bit of a halo around the edges instead of being crisply clear.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 5.4*10^9
Pitt Freezer Box# 106
Pitt Freezer Box Grid# E9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload