Arthrobacter phage Cuervo
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| Detailed Information for Phage Cuervo | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Lucia Soto Herrera |
| Year Found | 2019 |
| Location Found | Chandler, AZ United States |
| Finding Institution | Arizona State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 37°C |
| GPS Coordinates | 33.420621 N, 111.932855 W Map |
| Discovery Notes | This phage was discovered in the soil of a garden pot that had a plant growing out of it. It was partially in the shade but received a decent amount of sunlight. The soil looked to be slightly moist. The garden pot was in a small garden that birds and stray cats are known to frequent so it is unknown albeit possible for there to be animal contact to the soil. The phage was thought to infect A. globiformis but it was discovered that the bacteria that the class had been using all along was actually a contaminant that had been isolated from the very first streak plate of the HHMI stock supply provided. FOR GEL OF ENZYME DIGESTS: (left to right) ladder, uncut, NspI, SacI, BamHI,ClaI NspI is the only one that cut the DNA |
| Naming Notes | The Spanish word for Crow since crow was already taken. I am absolutely fascinated by corvid studies and how intelligent these birds are. They can easily be trained to accomplish goals or understand human machines/patterns and I feel like that's part of what we do with phage. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | Based on plaques appears to be in lytic phase |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques are small (about the size of a pinprick) and appear clear, however given time to incubate they expand to be about the size of a #2 pencil's eraser. The larger plaques do appear to have a bit of a halo around the edges instead of being crisply clear. |
| Morphotype | Myoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 5.4*10^9 |
| Pitt Freezer Box# | 106 |
| Pitt Freezer Box Grid# | E9 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
