The Actinobacteriophage Database

Gordonia phage Culver

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Detailed Information for Phage Culver
Discovery Information
Isolation Host	Gordonia terrae CAG3
Found By	Brandon Stamey
Year Found	2017
Location Found	Sylva, NC USA
Finding Institution	Western Carolina University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Unknown
Isolation Temperature	Not entered
GPS Coordinates	35.3167 N, 83.1648 W Map
Discovery Notes	This sample was found within chicken coop compost
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jul 21, 2020
Sequencing Facility	Western Carolina University
Shotgun Sequencing Method	Illumina
Approximate Shotgun Coverage	344
Genome length (bp)	93062
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	CGCGACGCTC
GC Content	61.9%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	CQ
Subcluster	CQ1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Abscondus (CQ1) Aphelion (CQ1) Bachita (CQ1) ClubL (CQ1) Cucurbita (CQ1) Culver (CQ1) Dusty (CQ1) Engineer (CQ1) Geeche (CQ1) Lozinak (CQ1) Miskis (CQ1) Norvs (CQ1) PhinkBoden (CQ1) Smoothie (CQ1) Toniann (CQ1) WilliamBoone (CQ1) BrutonGaster (CQ2) OneUp (CQ2)
Annotating Institution	University of North Carolina at Charlotte
Annotation Status	In GenBank
Plaque Notes	This phage makes moderately sized, turgid, and uniform plaqes when spotted Also, this phage has a long tail (~450nm) and moderately sized capsid (79.3nm)
Morphotype	Siphoviridae
Number of Genes	182
Number of tRNAs	8
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Culver_1 Culver_2 Culver_3 Culver_4 Culver_5 Culver_6 Culver_7 Culver_8 Culver_9 Culver_10 Culver_11 Culver_12 Culver_13 (HNH endonuclease) Culver_14 Culver_15 Culver_16 Culver_17 Culver_18 Culver_19 — tRNA (tRNA-?(Val\|Arg)(gacg)) Culver_20 — tRNA (tRNA-Undet(nnn)) Culver_21 — tRNA (tRNA-Trp(cca)) Culver_22 — tRNA (tRNA-Tyr(gta)) Culver_23 — tRNA (tRNA-Ser(cga)) Culver_24 — tRNA (tRNA-Ser(gct)) Culver_25 Culver_26 — tRNA (tRNA-Asn(gtt)) Culver_27 Culver_28 — tRNA (tRNA-Lys(ctt)) Culver_29 (terminase, small subunit) Culver_30 (terminase, large subunit) Culver_31 (portal protein) Culver_32 Culver_33 (scaffolding protein) Culver_34 (capsid decoration protein, LamD-like) Culver_35 (major capsid protein) Culver_36 Culver_37 (head-to-tail adaptor) Culver_38 (head-to-tail stopper) Culver_39 Culver_40 (tail terminator) Culver_41 (endonuclease VII) Culver_42 (major tail protein) Culver_43 Culver_44 (tail assembly chaperone) Culver_45 (tail assembly chaperone) Culver_46 (tape measure protein) Culver_47 (minor tail protein) Culver_48 (minor tail protein) Culver_49 (lysin A, protease C39 domain) Culver_50 (lysin A, L-Ala-D-Glu peptidase domain) Culver_51 (holin) Culver_52 Culver_53 Culver_54 Culver_55 (minor tail protein) Culver_56 Culver_57 Culver_58 (minor tail protein) Culver_59 Culver_60 (tyrosine integrase) Culver_61 (minor tail protein) Culver_62 (Lsr2-like DNA bridging protein) Culver_63 Culver_64 Culver_65 (exonuclease) Culver_66 (exonuclease) Culver_67 Culver_68 Culver_69 Culver_70 Culver_71 (metallophosphoesterase) Culver_72 Culver_73 Culver_74 (rtcB-like RNA ligase) Culver_75 Culver_76 Culver_77 Culver_78 Culver_79 Culver_80 Culver_81 Culver_82 Culver_83 Culver_84 Culver_85 (tyrosine integrase) Culver_86 Culver_87 (immunity repressor) Culver_88 Culver_89 (RecE-like exonuclease) Culver_90 Culver_91 Culver_92 (RecT-like DNA pairing protein) Culver_93 Culver_94 Culver_95 (ThyX-like thymidylate synthase) Culver_96 Culver_97 Culver_98 Culver_99 Culver_100 (helix-turn-helix DNA binding domain) Culver_101 Culver_102 Culver_103 Culver_104 (helix-turn-helix DNA binding domain) Culver_105 (RepA-like replication initiator) Culver_106 (helicase loader) Culver_107 Culver_108 Culver_109 Culver_110 Culver_111 Culver_112 Culver_113 Culver_114 Culver_115 Culver_116 Culver_117 Culver_118 Culver_119 Culver_120 Culver_121 Culver_122 Culver_123 (DNA primase/helicase) Culver_124 Culver_125 (NrdH-like glutaredoxin) Culver_126 (SSB protein) Culver_127 Culver_128 (WhiB family transcription factor) Culver_129 Culver_130 Culver_131 Culver_132 Culver_133 (endonuclease VII) Culver_134 Culver_135 Culver_136 (nucleotidyl transferase) Culver_137 Culver_138 Culver_139 Culver_140 Culver_141 Culver_142 Culver_143 Culver_144 (5' nucleotidase) Culver_145 Culver_146 (deoxycytidylate deaminase) Culver_147 Culver_148 (lysin B) Culver_149 Culver_150 Culver_151 Culver_152 (helix-turn-helix DNA binding domain) Culver_153 Culver_154 Culver_155 Culver_156 Culver_157 Culver_158 Culver_159 Culver_160 Culver_161 Culver_162 Culver_163 Culver_164 Culver_165 Culver_166 Culver_167 Culver_168 Culver_169 Culver_170 Culver_171 Culver_172 Culver_173 Culver_174 Culver_175 Culver_176 Culver_177 Culver_178 Culver_179 Culver_180 Culver_181 Culver_182 Culver_183 Culver_184 Culver_185 Culver_186 Culver_187 Culver_188 Culver_189 Culver_190
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	OR475265
Refseq Number	None yet
Published in Paper	Alexander et al 2023, Microbiology Resource Announcements: From compost to culver: genome sequence and annotation of a cluster CQ1 Gordonia phage
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	68
Pitt Freezer Box Grid#	H4
Available Files
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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