Microbacterium phage Damascus
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Detailed Information for Phage Damascus
Discovery Information
Isolation HostMicrobacterium paraoxydans NRRL B-14843
Found ByFaith Cour
Year Found2022
Location FoundAmery, WI USA
Finding InstitutionUniversity of Wisconsin-River Falls
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature29°C
GPS Coordinates45.311936 N, 92.366226 W Map
Discovery Notes-I adopted a soil sample from Kaylee Yzermans
-The soil was loose, some parts moist but mostly dry soil
-The soil was taken on a hill going down to a lake
-Weather was partially cloudy, 72 degrees
Naming NotesThe name "Damascus" is the capital of Syria. Damascus is an important biblical reference during the life of Jesus before the official boundaries of Syria were created. Damascus is also unique and sounds cool.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 23, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage1197
Genome length (bp)56477
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCGTCACT
GC Content56.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEL
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members
Annotating InstitutionUniversity of Wisconsin-River Falls
Annotation StatusIn GenBank
Plaque NotesPlaques are about 1 mm in size, clear, uniform, spread out fairly evenly, no halos
MorphotypeSiphoviridae
Number of Genes82
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

Click to View

Damascus_1

Damascus_2
(endolysin)

Damascus_3

Damascus_4

Damascus_5

Damascus_6

Damascus_7
(HNH endonuclease)

Damascus_8

Damascus_9

Damascus_10
(terminase)

Damascus_11

Damascus_12
(portal protein)

Damascus_13

Damascus_14
(major capsid and protease fusion protein)

Damascus_15

Damascus_16
(tail terminator)

Damascus_17
(major tail protein)

Damascus_18

Damascus_19
(major tail protein)

Damascus_20
(major tail protein)

Damascus_21

Damascus_22

Damascus_23

Damascus_24
(tape measure protein)

Damascus_25
(minor tail protein)

Damascus_26
(minor tail protein)

Damascus_27

Damascus_28
(minor tail protein)

Damascus_29

Damascus_30

Damascus_31

Damascus_32

Damascus_33

Damascus_34
(acetyltransferase)

Damascus_35

Damascus_36

Damascus_37

Damascus_38

Damascus_39

Damascus_40

Damascus_41

Damascus_42

Damascus_43

Damascus_44

Damascus_45

Damascus_46

Damascus_47

Damascus_48

Damascus_49

Damascus_50
(DNA primase/polymerase)

Damascus_51

Damascus_52

Damascus_53

Damascus_54

Damascus_55

Damascus_56

Damascus_57

Damascus_58
(HNH endonuclease)

Damascus_59
(HNH endonuclease)

Damascus_60

Damascus_61
(DNA helicase)

Damascus_62

Damascus_63
(MazG-like pyrophosphohydrolase)

Damascus_64

Damascus_65

Damascus_66

Damascus_67

Damascus_68
(ThyX-like thymidylate synthase)

Damascus_69

Damascus_70

Damascus_71

Damascus_72

Damascus_73

Damascus_74

Damascus_75

Damascus_76

Damascus_77

Damascus_78

Damascus_79
(VRR-Nuc domain protein)

Damascus_80
(Cas4-family exonuclease)

Damascus_81
(helix-turn-helix DNA binding domain protein)

Damascus_82

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOQ995438
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 155
Pitt Freezer Box Grid# D8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload