Gordonia phage Dardanus
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Detailed Information for Phage Dardanus
Discovery Information
Isolation HostGordonia terrae 3612
Found BySamantha J. Wills
Year Found2016
Location FoundFort Myers, FL USA
Finding InstitutionFlorida Gulf Coast University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates26.460278 N, 81.78 W Map
Discovery NotesMy bacteriophage was found in the Food Forest at Florida Gulf Coast University. The soil was dry and sandy. My bacteriophage was both enriched and plated on the bacteria Gordonia terrae.
Naming NotesMy bacteriophage is named after the Greek Demigod Dardanus. Dardanus is the son of Zeus and Electra, and the grandson of Atlas according to Greek mythology. This name is meant to reflect my bacteriophage's power. I view my bacteriophage as powerful because it covered my entire plate at the beginning of a serial dilution series.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 16, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage278
Genome length (bp)43143
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCCCGGCAGGTA
GC Content66.6%
Sequencing NotesThe sequencing reads from this phage showed that there were at least two deletions in a subset of the population, including one that was present in more than half of the population. The final fasta file that is posted contains these deleted regions, even though the first one is present in a minority of the actual phage particles.

Deletion #1 is found in about 70% of the reads, and is a 1218 bp deletion from 25156 to 26373, inclusive.

Deletion #2 is found in about 20% of the reads that do NOT have deletion #1, and is 901 bp from 25320 to 26220, inclusive.
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Lysogeny NotesMy plaques were round and clear.
Other Cluster Members
Annotating InstitutionUniversity of Louisiana at Monroe
Annotation StatusIn GenBank
Plaque NotesMy phage came from a lyctic plaque inside a turbid plaque. When plated on gordonia terrae for a serial dillution series, small clear plaques developed, as well as large turbid plaques. The least amount of plaques found on a plate was 10 plaques. My plaques are large, however they vary in size. My plaques have a diameter of approximately 2-3.5 millimeters.
Has been Phamerated?Yes
Gene List

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Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN010758
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 44
Pitt Freezer Box Grid# G10
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload