Mycobacterium phage Diliz
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Detailed Information for Phage Diliz
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found By03/06
Year Found2017
Location FoundGreensburg , PA USA
Finding InstitutionSeton Hill University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS CoordinatesUnavailable
Discovery NotesHeavily pedestrianized area containing a fair amount of litter and rock chippings, all of which were unnatural and may have caused contamination. The soil sample was extracted from the ground surface. However, due to the rain on the day of the data collection, the soil was very waterlogged. This again might have influenced the soil and therefore the results.
Naming NotesIn the name of people who found Diliz. Diana Carvajalino and Elizabeth Ginter
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque Notesthe morphology and purification of the bacteriophage Diliz as result of a serial dilution performed using the collected lysate. As shown above, the plaques look cloudy, which means that the plaques are turbid. Therefore, it is a temperate phage. A temperate phage contains killed cells with the company of lysogens. The uniform size of the plaques is also shown above; this means a bacteriophage was isolated. Mycobacterium smegmatis was used as a host for Diliz on a 7H9 top agar plate. The plate was incubated five days at 30°C. 95 plaques were counted on the plate 10-1, this means that it was a countable plate. A countable plate is a plate that has between 20-200 plaques
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 59
Pitt Freezer Box Grid# F8