Mycobacterium phage Diliz
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Detailed Information for Phage Diliz | |
Discovery Information | |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | 03/06 |
Year Found | 2017 |
Location Found | Greensburg , PA USA |
Finding Institution | Seton Hill University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | Unavailable |
Discovery Notes | Heavily pedestrianized area containing a fair amount of litter and rock chippings, all of which were unnatural and may have caused contamination. The soil sample was extracted from the ground surface. However, due to the rain on the day of the data collection, the soil was very waterlogged. This again might have influenced the soil and therefore the results. |
Naming Notes | In the name of people who found Diliz. Diana Carvajalino and Elizabeth Ginter |
Sequencing Information | |
Sequencing Complete? | No |
Genome length (bp) | Unknown |
Character of genome ends | Unknown |
Fasta file available? | No |
Characterization | |
Cluster | Unclustered |
Subcluster | -- |
Annotating Institution | Unknown or unassigned |
Annotation Status | Not sequenced |
Plaque Notes | the morphology and purification of the bacteriophage Diliz as result of a serial dilution performed using the collected lysate. As shown above, the plaques look cloudy, which means that the plaques are turbid. Therefore, it is a temperate phage. A temperate phage contains killed cells with the company of lysogens. The uniform size of the plaques is also shown above; this means a bacteriophage was isolated. Mycobacterium smegmatis was used as a host for Diliz on a 7H9 top agar plate. The plate was incubated five days at 30°C. 95 plaques were counted on the plate 10-1, this means that it was a countable plate. A countable plate is a plate that has between 20-200 plaques |
Has been Phamerated? | No |
Publication Info | |
Uploaded to GenBank? | No |
GenBank Accession | None yet |
Refseq Number | None yet |
Archiving Info | |
Archiving status | Archived |
Pitt Freezer Box# | 59 |
Pitt Freezer Box Grid# | F8 |