| Detailed Information for Phage DonTron | |
| Discovery Information | |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Alyssa Williams |
| Year Found | 2023 |
| Location Found | New Albany, IN United States |
| Finding Institution | Indiana University-Southeast |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 26°C |
| GPS Coordinates | 38.34724 N, 85.82167 W Map |
| Discovery Notes | Alyssa found this sample location on the Bicentennial trail on campus. Its exact GPS coordinates are 38.347.24 degrees north, and 85.82167 degrees west. It was 90 degrees Fahrenheit outside and sunny. The soil was dug from about 2 inches in the ground. It was by a freshly fallen tree. The soil was dry and loose. It contained roots and tiny twigs and was light brown with a sandy texture |
| Naming Notes | "DonTron" came from a TV show called Rise: of the TMNT. It is a character named Donnie's nickname in the show, and we thought that it would be a good name for our phage instead of just our initials. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 2, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 163 |
| Genome length (bp) | 58182 |
| Character of genome ends | Circularly Permuted |
| GC Content | 65.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | CP |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members | |
| Annotating Institution | University of Lagos |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques of our phage are transparent and range from 1mm –2mm in diameter. We have a lot of variability between our plaques. For example, we have some that are more rectangular with rough edges and some that are completely round with even edges. |
| Number of Genes | 91 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewDonTron_1 DonTron_2 DonTron_3 DonTron_4 DonTron_5 DonTron_6 DonTron_7 DonTron_8 DonTron_9 DonTron_10 DonTron_11 DonTron_12 DonTron_13 DonTron_14 DonTron_15 DonTron_16 DonTron_17 DonTron_18 DonTron_19 DonTron_20 DonTron_21 DonTron_22 DonTron_23 DonTron_24 DonTron_25 DonTron_26 DonTron_27 DonTron_28 DonTron_29 DonTron_30 DonTron_31 DonTron_32 DonTron_33 DonTron_34 DonTron_35 DonTron_36 DonTron_37 DonTron_38 DonTron_39 DonTron_40 DonTron_41 DonTron_42 DonTron_43 DonTron_44 DonTron_45 DonTron_46 DonTron_47 DonTron_48 DonTron_49 DonTron_50 DonTron_51 DonTron_52 DonTron_53 DonTron_54 DonTron_55 DonTron_56 DonTron_57 DonTron_58 DonTron_59 DonTron_60 DonTron_61 DonTron_62 DonTron_63 DonTron_64 DonTron_65 DonTron_66 DonTron_67 DonTron_68 DonTron_69 DonTron_70 DonTron_71 DonTron_72 DonTron_73 DonTron_74 DonTron_75 DonTron_76 DonTron_77 DonTron_78 DonTron_79 DonTron_80 DonTron_81 DonTron_82 DonTron_83 DonTron_84 DonTron_85 DonTron_86 DonTron_87 DonTron_88 DonTron_89 DonTron_90 DonTron_91 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PX315707 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 184 |
| Pitt Freezer Box Grid# | A1 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |