| Detailed Information for Phage DreamEater | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | H. Kepner,H. Roadman,S. Lewis,E. Shaffer,F. Boyle |
| Year Found | 2024 |
| Location Found | Mexico, PA United States |
| Finding Institution | University of Pittsburgh at Greensburg |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.53865 N, 77.35832 W Map |
| Discovery Notes | Soil sample was found under a locus tree from an old horseshoe pit. |
| Naming Notes | Hunter Roadman saw in a dream that our second purification of our isolate would come back with no phage the night prior to seeing the result. When our group returned to the lab to check, it originally appeared to have no phage, but upon further investigation and help from our instructor we found extremely hazy and large plaques. As they were exceedingly hard to see, we believe that they were "eaten by the dream", thus giving the name DreamEater. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 9, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 194 |
| Genome length (bp) | 46190 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | FJ |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Lysogeny Notes | Initial plaques produced were remarkably turbid, with a distinctive morphology at 48 hours incubation at 30°C. Interestingly, when plates were incubated at 30°C for 120 hours plaques exhibited a marked decrease in turbidity, suggesting a longer period between initial infection and eventual lysis of the host bacteria within the bacterial lawn; due to these findings we infer that Dreameater functions as a lysogenic bacteriophage. |
| Other Cluster Members |
Click to View |
| Annotating Institution | University of Pittsburgh at Greensburg |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | Turbid, hazy, bullseye, medium sized plaques. |
| Number of Genes | 83 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewDreamEater_1 DreamEater_2 DreamEater_3 DreamEater_4 DreamEater_5 DreamEater_6 DreamEater_7 DreamEater_8 DreamEater_9 DreamEater_10 DreamEater_11 DreamEater_12 DreamEater_13 DreamEater_14 DreamEater_15 DreamEater_16 DreamEater_17 DreamEater_18 DreamEater_19 DreamEater_20 DreamEater_21 DreamEater_22 DreamEater_23 DreamEater_24 DreamEater_25 DreamEater_26 DreamEater_27 DreamEater_28 DreamEater_29 DreamEater_30 DreamEater_31 DreamEater_32 DreamEater_33 DreamEater_34 DreamEater_35 DreamEater_36 DreamEater_37 DreamEater_38 DreamEater_39 DreamEater_40 DreamEater_41 DreamEater_42 DreamEater_43 DreamEater_44 DreamEater_45 DreamEater_46 DreamEater_47 DreamEater_48 DreamEater_49 DreamEater_50 DreamEater_51 DreamEater_52 DreamEater_53 DreamEater_54 DreamEater_55 DreamEater_56 DreamEater_57 DreamEater_58 DreamEater_59 DreamEater_60 DreamEater_61 DreamEater_62 DreamEater_63 DreamEater_64 DreamEater_65 DreamEater_66 DreamEater_67 DreamEater_68 DreamEater_69 DreamEater_70 DreamEater_71 DreamEater_72 DreamEater_73 DreamEater_74 DreamEater_75 DreamEater_76 DreamEater_77 DreamEater_78 DreamEater_79 DreamEater_80 DreamEater_81 DreamEater_82 DreamEater_83 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 195 |
| Pitt Freezer Box Grid# | E2 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
