Arthrobacter phage DreamEater
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| Detailed Information for Phage DreamEater | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | H. Kepner,H. Roadman,S. Lewis,E. Shaffer,F. Boyle |
| Year Found | 2024 |
| Location Found | Mexico, PA United States |
| Finding Institution | University of Pittsburgh at Greensburg |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.53865 N, 77.35832 W Map |
| Discovery Notes | Soil sample was found under a locus tree from an old horseshoe pit. |
| Naming Notes | Hunter Roadman saw in a dream that our second purification of our isolate would come back with no phage the night prior to seeing the result. When our group returned to the lab to check, it originally appeared to have no phage, but upon further investigation and help from our instructor we found extremely hazy and large plaques. As they were exceedingly hard to see, we believe that they were "eaten by the dream", thus giving the name DreamEater. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 9, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 194 |
| Genome length (bp) | 46190 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | FJ |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Lysogeny Notes | Initial plaques produced were remarkably turbid, with a distinctive morphology at 48 hours incubation at 30°C. Interestingly, when plates were incubated at 30°C for 120 hours plaques exhibited a marked decrease in turbidity, suggesting a longer period between initial infection and eventual lysis of the host bacteria within the bacterial lawn; due to these findings we infer that Dreameater functions as a lysogenic bacteriophage. |
| Other Cluster Members |
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| Annotating Institution | University of Pittsburgh at Greensburg |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | Turbid, hazy, bullseye, medium sized plaques. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 195 |
| Pitt Freezer Box Grid# | E2 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
