| Detailed Information for Phage Dryad | |
| Discovery Information | |
| Isolation Host | Streptomyces viridochromogenes DSM40736 |
| Found By | Marta Taye and Iris Chin |
| Year Found | 2015 |
| Location Found | Eureka, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 27°C |
| GPS Coordinates | 38.51817 N, 90.556374 W Map |
| Discovery Notes | Our soil was collected from the forest surrounding the Washington University Tyson Research Center on 8/29/15. The collection site was on southward-facing hill, far from any roads or other human structures. The soil was collected at a depth of 4cm after the leaf litter which rested on top of it was cleared away. The weather at time of collection (1:30PM) was partly cloudy. |
| Naming Notes | From Greek Mythology: a dryad is a nymph of the forest. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 18, 2019 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 130 |
| Genome length (bp) | 68994 |
| Character of genome ends | Circularly Permuted |
| GC Content | 63.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BN |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | Washington University in St. Louis |
| Annotation Status | In GenBank |
| Plaque Notes | Despite having done 5 rounds of purification, the plaques vary greatly in size (from barely visible to about 2mm in diameter). They are circular and fully clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 109 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewDryad_1 Dryad_2 Dryad_3 Dryad_4 Dryad_5 Dryad_6 Dryad_7 Dryad_8 Dryad_9 Dryad_10 Dryad_11 Dryad_12 Dryad_13 Dryad_14 Dryad_15 Dryad_16 Dryad_17 Dryad_18 Dryad_19 Dryad_20 Dryad_21 Dryad_22 Dryad_23 Dryad_24 Dryad_25 Dryad_26 Dryad_27 Dryad_28 Dryad_29 Dryad_30 Dryad_31 Dryad_32 Dryad_33 Dryad_34 Dryad_35 Dryad_36 Dryad_37 Dryad_38 Dryad_39 Dryad_40 Dryad_41 Dryad_42 Dryad_43 Dryad_44 Dryad_45 Dryad_46 Dryad_47 Dryad_48 Dryad_49 Dryad_50 Dryad_51 Dryad_52 Dryad_53 Dryad_54 Dryad_55 Dryad_56 Dryad_57 Dryad_58 Dryad_59 Dryad_60 Dryad_61 Dryad_62 Dryad_63 Dryad_64 Dryad_65 Dryad_66 Dryad_67 Dryad_68 Dryad_69 Dryad_70 Dryad_71 Dryad_72 Dryad_73 Dryad_74 Dryad_75 Dryad_76 Dryad_77 Dryad_78 Dryad_79 Dryad_80 Dryad_81 Dryad_82 Dryad_83 Dryad_84 Dryad_85 Dryad_86 Dryad_87 Dryad_88 Dryad_89 Dryad_90 Dryad_91 Dryad_92 Dryad_93 Dryad_94 Dryad_95 Dryad_96 Dryad_97 Dryad_98 Dryad_99 Dryad_100 Dryad_101 Dryad_102 Dryad_103 Dryad_104 Dryad_105 Dryad_106 Dryad_107 Dryad_108 Dryad_109 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT498037 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 1.98 x 10^8 pfu/mL |
| Date of SEA Lysate Titering | Oct 13, 2015 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
