Streptomyces phage Dryad
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Detailed Information for Phage Dryad
Discovery Information
Isolation HostStreptomyces viridochromogenes DSM40736
Found ByMarta Taye and Iris Chin
Year Found2015
Location FoundEureka, MO USA
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature27°C
GPS Coordinates38.51817 N, 90.556374 W Map
Discovery NotesOur soil was collected from the forest surrounding the Washington University Tyson Research Center on 8/29/15. The collection site was on southward-facing hill, far from any roads or other human structures. The soil was collected at a depth of 4cm after the leaf litter which rested on top of it was cleared away. The weather at time of collection (1:30PM) was partly cloudy.
Naming NotesFrom Greek Mythology: a dryad is a nymph of the forest.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 18, 2019
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage130
Genome length (bp)68994
Character of genome endsCircularly Permuted
GC Content63.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBN
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionWashington University in St. Louis
Annotation StatusBeing Annotated (Expected completion by 3/18/2020)
Plaque NotesDespite having done 5 rounds of purification, the plaques vary greatly in size (from barely visible to about 2mm in diameter). They are circular and fully clear.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 1.98 x 10^8 pfu/mL
Date of SEA Lysate Titering Oct 13, 2015
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload