Streptomyces phage Rooney
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Detailed Information for Phage Rooney
Discovery Information
Isolation HostStreptomyces viridochromogenes DSM40736
Found ByHannah Boozalis and Margaret Gaggioli
Year Found2015
Location FoundSt. Louis, MO USA
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature29°C
GPS Coordinates38.648 N, 90.306 W Map
Discovery NotesThe sample was taken from a muddy puddle next to a path in Brookings Quad on the Danforth Campus of Washington University in St. Louis.
Naming Notes'Rooney' comes from the name of the Dean of Students in "Ferris Bueller's Day Off".
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 20, 2020
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage165
Genome length (bp)69514
Character of genome endsCircularly Permuted
GC Content64.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBN
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionWashington University in St. Louis
Annotation StatusQC Complete
Plaque NotesThe plaques were relatively small in size and mostly circular in shape. They were clear and had defined edges. After purification they had an average radius of 1 mm.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Rooney_1
(terminase, small subunit)

Rooney_2

Rooney_3

Rooney_4

Rooney_5

Rooney_6

Rooney_7

Rooney_8
(terminase, large subunit)

Rooney_9
(portal protein)

Rooney_10

Rooney_11

Rooney_12

Rooney_13
(scaffolding protein)

Rooney_14
(major capsid protein)

Rooney_15
(head-to-tail adaptor)

Rooney_16
(head-to-tail stopper)

Rooney_17

Rooney_18

Rooney_19
(tail terminator)

Rooney_20
(major tail protein)

Rooney_21
(tail assembly chaperone)

Rooney_22
(tail assembly chaperone)

Rooney_23
(tape measure protein)

Rooney_24
(minor tail protein)

Rooney_25
(minor tail protein)

Rooney_26
(minor tail protein)

Rooney_27

Rooney_28
(endolysin)

Rooney_29

Rooney_30

Rooney_31
(glycosyltransferase)

Rooney_32
(nucleotidyl transferase)

Rooney_33
(methyltransferase)

Rooney_34

Rooney_35
(DNA helicase)

Rooney_36
(RuvC-like resolvase)

Rooney_37

Rooney_38

Rooney_39
(HNH endonuclease)

Rooney_40

Rooney_41

Rooney_42

Rooney_43

Rooney_44

Rooney_45
(nkf)

Rooney_46
(ThyX-like thymidylate synthase)

Rooney_47

Rooney_48

Rooney_49

Rooney_50

Rooney_51
(DnaE-like DNA polymerase III (alpha))

Rooney_52

Rooney_53

Rooney_54
(phosphatase)

Rooney_55
(oxidoreductase)

Rooney_56

Rooney_57

Rooney_58

Rooney_59

Rooney_60

Rooney_61

Rooney_62
(tellurium resistance protein D family)

Rooney_63

Rooney_64
(RecA-like DNA recombinase)

Rooney_65

Rooney_66
(Cas4 family exonuclease)

Rooney_67

Rooney_68

Rooney_69

Rooney_70
(helicase)

Rooney_71
(deoxycytidylate deaminase)

Rooney_72

Rooney_73

Rooney_74

Rooney_75

Rooney_76

Rooney_77

Rooney_78

Rooney_79

Rooney_80

Rooney_81

Rooney_82

Rooney_83

Rooney_84
(helix-turn-helix DNA binding domain)

Rooney_85

Rooney_86

Rooney_87

Rooney_88

Rooney_89
(helix-turn-helix DNA binding domain)

Rooney_90

Rooney_91

Rooney_92

Rooney_93

Rooney_94

Rooney_95

Rooney_96

Rooney_97

Rooney_98

Rooney_99

Rooney_100

Rooney_101

Rooney_102

Rooney_103
(DNA primase/helicase)

Rooney_104

Rooney_105

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Designator Rooney
SEA Lysate Titer 1.43x10^10 pfu/ml
Date of SEA Lysate Titering Oct 8, 2015
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload