Streptomyces phage FrankieMermaid
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| Detailed Information for Phage FrankieMermaid | |
| Discovery Information | |
| Isolation Host | Streptomyces lividans NRRL B-12275 |
| Found By | Katherine Morton |
| Year Found | 2025 |
| Location Found | Arlington, TX United States |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 32.730056 N, 97.164444 W Map |
| Discovery Notes | I collected soil from West Arlington, Texas at 4:30pm. The temperature at that time in the afternoon was 96F with 43% humidity. The location was in a flowerbed to the right of my yard facing the house, next to a tree. I dug approximately 1.5 inches below the surface, collected the sample, and placed it into a plastic bag. |
| Naming Notes | I am a huge fan of silly movies and one of my favorite silly movies and one of my favorite movies ever has a character named Frankie Mermaid and I think the silly name is pretty fitting for my Phage |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | I obtained 5 plates and labeled them with the name of the phage, the name of the host bacterium, and one of the following five dilutions: 10^2, 10^3, 10^4, 10^5, 10^6. I then added 100μl of my high titer lysate phage sample to each of the plates and spread them evenly. The TA then provided me with the dilution series of host bacteria. I then added 100μl of host bacteria to the corresponding dilution (100μl of the 10^2 dilution to the plate labeled 10^2) and spread the solution evenly on top. The plates were then incubated for the duration it takes colonies to form (typically 2-5 days) Results were negative |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | 1.0 milliliters in diameter, round, medium sized |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
