| Discovery Information |
| Isolation Host | Streptomyces mirabilis NRRL B-2400 |
| Former names | Sheryl |
| Found By | Driwech,Oumaima & Fritz,Lily |
| Year Found | 2023 |
| Location Found | Catonsville, MD USA |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.25536 N, 76.711944 W Map |
| Discovery Notes | Soil was collected on 10/24/2023 outside of the UMBC Biology building exit door. The ambient temperature was 18.3 C. Soil was dug around 3-4 inches below the surface. The soil was collected by Abdullah Bajwah and Zain Naeem |
| Naming Notes | We chose the name GiJojo because we had to have a lot of time and patience to get his phage and it was very resilient. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 19, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 235 |
| Genome length (bp) | 115161 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 197 bp |
| GC Content | 53.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BS |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Lysogeny Notes | Lysogeny testing was not performed. |
| Other Cluster Members |
|
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | In GenBank |
| Plaque Notes | Clear, circular, smooth edges, symmetrical |
| Morphotype | Myoviridae |
| Number of Genes | 180 |
| Number of tRNAs | 29 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP725410 |
| Refseq Number | None yet |
| Published in Paper | Badiola et al 2024, Microbiology Resource Announcements: Genome sequence of bacteriophage GiJojo, isolated using Streptomyces mirabilis in Catonsville, Maryland |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
171 |
| Pitt Freezer Box Grid# |
C1 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
