| Detailed Information for Phage Greenbelt |
| Discovery Information |
| Isolation Host | Streptomyces venezuelae ATCC 10712 |
| Found By | Ava Mattox |
| Year Found | 2024 |
| Location Found | Wylie, TX United States |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 32.98183 N, 96.54667 W Map |
| Discovery Notes | Soil was collected on 8/18/24 from creekside mud on a greenbelt in Wylie, Texas. It was collected at 2:14 p.m., and the temperature was 100 degrees Fahrenheit. |
| Naming Notes | Selected "Greenbelt" as the initial soil sample was collected on a greenbelt. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 8, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 3692 |
| Genome length (bp) | 66149 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BN |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Positive lysogeny results down to 10^-7, with -8 not forming a complete mesa but small plaques are visible. |
| Other Cluster Members |
|
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | Clear, circular, 1mm diameter, some plaques with signs of turbidity. |
| Morphotype | Siphoviridae |
| Number of Genes | 105 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PV915898 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
204 |
| Pitt Freezer Box Grid# |
H12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
