Gordonia phage Guey18
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Detailed Information for Phage Guey18
Discovery Information
Isolation HostGordonia terrae 3612
Found ByChiara Rigaud
Year Found2018
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.4413 N, 79.9534 W Map
Discovery NotesThis soil was collected in an area between my Universities Library ( Hillman Library) and Posvar Hall. The soil was found in a shrub closer to Hillman, where people can sit on a bench near the shade. A torrential downpour had occurred when I collected my sample, so the soil was quite wet/moist.
Naming NotesI chose the name Güey18 because it's one of my favorite words in Spanish when referring to someone. It's a word I love and use quite frequently when I'm back home, so in a sense, it reminds me of home.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 10, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage472
Genome length (bp)68896
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceACTCTGCCCAT
GC Content50.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterDP
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionNorth Carolina A&T State University
Annotation StatusIn GenBank
Plaque NotesMy plaque was clear making it more lytic/ virulent rather than temperate. The plaque was medium in size.
MorphotypeSiphoviridae
Number of Genes149
Number of tRNAs2
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Guey18_1

Guey18_2

Guey18_3

Guey18_4

Guey18_5

Guey18_6

Guey18_7

Guey18_8

Guey18_9

Guey18_10

Guey18_11

Guey18_12

Guey18_13

Guey18_14

Guey18_15

Guey18_16

Guey18_17

Guey18_18

Guey18_19

Guey18_20

Guey18_21

Guey18_22

Guey18_23

Guey18_24

Guey18_25

Guey18_26

Guey18_27

Guey18_28

Guey18_29

Guey18_30

Guey18_31
(Lsr2-like DNA bridging protein)

Guey18_32
(serine hydrolase)

Guey18_33

Guey18_34

Guey18_35

Guey18_36

Guey18_37

Guey18_38

Guey18_39
(terminase)

Guey18_40
(portal protein)

Guey18_41
(capsid maturation protease)

Guey18_42

Guey18_43
(scaffolding protein)

Guey18_44
(major capsid protein)

Guey18_45
(head-to-tail adaptor)

Guey18_46
(head-to-tail stopper)

Guey18_47

Guey18_48

Guey18_49
(tail terminator)

Guey18_50
(major tail protein)

Guey18_51
(tail assembly chaperone)

Guey18_52
(tail assembly chaperone)

Guey18_53
(AAA-ATPase)

Guey18_54

Guey18_55

Guey18_56
(tape measure protein)

Guey18_57
(minor tail protein)

Guey18_58
(minor tail protein)

Guey18_59

Guey18_60
(minor tail protein)

Guey18_61

Guey18_62

Guey18_63

Guey18_64

Guey18_65

Guey18_66
(lysin A)

Guey18_67
(holin)

Guey18_68

Guey18_69

Guey18_70
(Lsr2-like DNA bridging protein)

Guey18_71

Guey18_72

Guey18_73

Guey18_74

Guey18_75

Guey18_76

Guey18_77

Guey18_78

Guey18_79

Guey18_80

Guey18_81

Guey18_82

Guey18_83

Guey18_84

Guey18_85

Guey18_86

Guey18_87
(Cas4 family exonuclease)

Guey18_88

Guey18_89

Guey18_90

Guey18_91

Guey18_92

Guey18_93

Guey18_94

Guey18_95

Guey18_96
(WhiB family transcription factor)

Guey18_97
(helix-turn-helix DNA-binding domain protein)

Guey18_98
(WhiB family transcription factor)

Guey18_99

Guey18_100
(HNH endonuclease)

Guey18_101

Guey18_102

Guey18_103

Guey18_104

Guey18_105

Guey18_106
(DnaB-like dsDNA helicase)

Guey18_107
(ssDNA binding protein)

Guey18_108

Guey18_109

Guey18_110

Guey18_111

Guey18_112
(endonuclease VII)

Guey18_113

Guey18_114

Guey18_115

Guey18_116
(ERF family ssDNA binding protein)

Guey18_117

Guey18_118

Guey18_119

Guey18_120
(helix-turn-helix DNA-binding domain protein)

Guey18_121
(NrdH-like glutaredoxin)

Guey18_122

Guey18_123
(MRE11 double-strand break endo/exonuclease)

Guey18_124

Guey18_125
(glycosyltransferase)

Guey18_126

Guey18_127

Guey18_128

Guey18_129 — tRNA
(tRNA-Asn(gtt))

Guey18_130 — tRNA
(tRNA-Leu(tag))

Guey18_131

Guey18_132

Guey18_133

Guey18_134

Guey18_135

Guey18_136

Guey18_137

Guey18_138

Guey18_139

Guey18_140

Guey18_141

Guey18_142

Guey18_143
(glycosyltransferase)

Guey18_144

Guey18_145

Guey18_146

Guey18_147

Guey18_148

Guey18_149
(HNH endonuclease)

Guey18_150

Guey18_151

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMW601220
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.07E12 pfu/mL
Pitt Freezer Box# 90
Pitt Freezer Box Grid# C2
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload