| Detailed Information for Phage Guey18 |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Chiara Rigaud |
| Year Found | 2018 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.4413 N, 79.9534 W Map |
| Discovery Notes | This soil was collected in an area between my Universities Library ( Hillman Library) and Posvar Hall. The soil was found in a shrub closer to Hillman, where people can sit on a bench near the shade. A torrential downpour had occurred when I collected my sample, so the soil was quite wet/moist. |
| Naming Notes | I chose the name Güey18 because it's one of my favorite words in Spanish when referring to someone. It's a word I love and use quite frequently when I'm back home, so in a sense, it reminds me of home. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 10, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 472 |
| Genome length (bp) | 68896 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | ACTCTGCCCAT |
| GC Content | 50.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DP |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | North Carolina A & T State University |
| Annotation Status | In GenBank |
| Plaque Notes | My plaque was clear making it more lytic/ virulent rather than temperate. The plaque was medium in size. |
| Morphotype | Siphoviridae |
| Number of Genes | 149 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW601220 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.07E12 pfu/mL |
| Pitt Freezer Box# |
90 |
| Pitt Freezer Box Grid# |
C2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
