Detailed Information for Phage Guey18 |
Discovery Information |
Isolation Host | Gordonia terrae 3612 |
Found By | Chiara Rigaud |
Year Found | 2018 |
Location Found | Pittsburgh, PA USA |
Finding Institution | University of Pittsburgh |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | No |
Isolation Temperature | 30°C |
GPS Coordinates | 40.4413 N, 79.9534 W Map |
Discovery Notes | This soil was collected in an area between my Universities Library ( Hillman Library) and Posvar Hall. The soil was found in a shrub closer to Hillman, where people can sit on a bench near the shade. A torrential downpour had occurred when I collected my sample, so the soil was quite wet/moist. |
Naming Notes | I chose the name Güey18 because it's one of my favorite words in Spanish when referring to someone. It's a word I love and use quite frequently when I'm back home, so in a sense, it reminds me of home. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Mar 10, 2020 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina |
Approximate Shotgun Coverage | 472 |
Genome length (bp) | 68896 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 11 bases |
Overhang Sequence | ACTCTGCCCAT |
GC Content | 50.1% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | DP |
Subcluster | -- |
Cluster Life Cycle | Lytic |
Other Cluster Members |
|
Annotating Institution | North Carolina A&T State University |
Annotation Status | In GenBank |
Plaque Notes | My plaque was clear making it more lytic/ virulent rather than temperate. The plaque was medium in size. |
Morphotype | Siphoviridae |
Number of Genes | 149 |
Number of tRNAs | 2 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MW601220 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
1.07E12 pfu/mL |
Pitt Freezer Box# |
90 |
Pitt Freezer Box Grid# |
C2 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
GenBank File for Phamerator | Download |