| Detailed Information for Phage Hiyaa |
| Discovery Information |
| Isolation Host | Streptomyces lividans JI 1326 |
| Found By | Zoe Tian, Julia Lieu, Maggie Kieser |
| Year Found | 2017 |
| Location Found | St. Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 30°C |
| GPS Coordinates | 38.6425 N, 90.3168 W Map |
| Discovery Notes | Outside temperature: 86 degrees F
Dirt texture: dry, organic matter
Depth of sample: 2 cm
Location description: boulevard between open ends of Forest Ridge Place |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 12, 2018 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 200 |
| Genome length (bp) | 83219 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BQ |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
|
| Annotating Institution | Washington University in St. Louis |
| Annotation Status | In GenBank |
| Plaque Notes | First round of purification: small plaques, round, clear.
After 3 rounds of purification: small plaques (pinprick - 1mm diameter), round, cloudy.
note: plaque picture uploaded is of first round of purification |
| Morphotype | Siphoviridae |
| Number of Genes | 124 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK279841 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.0*10^9 |
| Pitt Freezer Box# |
49 |
| Pitt Freezer Box Grid# |
C9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
