| Detailed Information for Phage HotPotato | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Kyla Douglas and Emma Medler |
| Year Found | 2024 |
| Location Found | Poughkeepsie, NY United States of America |
| Finding Institution | Marist University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 41.72052 N, 73.93549 W Map |
| Discovery Notes | It was found outside a dorm at Marist College, in a flower bed. The phage was obtained with a spoon and plastic bag within the dirt and mulch. |
| Naming Notes | In the soil we collected, a potato shaped rock was obtained. This rock was sitting with the soil even when we started the process to prepare for spot testing the sample, it even got stuck in the test tube at one point. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 16, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 5483 |
| Genome length (bp) | 42969 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGGAGA |
| GC Content | 64.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | FA |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | Marist University |
| Annotation Status | Being Annotated (Expected completion by 5/1/2025) |
| Plaque Notes | The plaques were uniform and clear in the center, fuzzier towards the outside. They were small and abundant. |
| Morphotype | Siphoviridae |
| Number of Genes | 65 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewHotPotato_1 HotPotato_2 HotPotato_3 HotPotato_4 HotPotato_5 HotPotato_6 HotPotato_7 HotPotato_8 HotPotato_9 HotPotato_10 HotPotato_11 HotPotato_12 HotPotato_13 HotPotato_14 HotPotato_15 HotPotato_16 HotPotato_17 HotPotato_18 HotPotato_19 HotPotato_20 HotPotato_21 HotPotato_22 HotPotato_23 HotPotato_24 HotPotato_25 HotPotato_26 HotPotato_27 HotPotato_28 HotPotato_29 HotPotato_30 HotPotato_31 HotPotato_32 HotPotato_33 HotPotato_34 HotPotato_35 HotPotato_36 HotPotato_37 HotPotato_38 HotPotato_39 HotPotato_40 HotPotato_41 HotPotato_42 HotPotato_43 HotPotato_44 HotPotato_45 HotPotato_46 HotPotato_47 HotPotato_48 HotPotato_49 HotPotato_50 HotPotato_51 HotPotato_52 HotPotato_53 HotPotato_54 HotPotato_55 HotPotato_56 HotPotato_57 HotPotato_58 HotPotato_59 HotPotato_60 HotPotato_61 HotPotato_62 HotPotato_63 HotPotato_64 HotPotato_65 — tRNA HotPotato_66 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 5x10^10 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
