Arthrobacter phage HotPotato
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Detailed Information for Phage HotPotato
Discovery Information
Isolation HostArthrobacter globiformis B-2979
Found ByKyla Douglas and Emma Medler
Year Found2024
Location FoundPoughkeepsie, NY United States of America
Finding InstitutionMarist University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates41.72052 N, 73.93549 W Map
Discovery NotesIt was found outside a dorm at Marist College, in a flower bed. The phage was obtained with a spoon and plastic bag within the dirt and mulch.
Naming NotesIn the soil we collected, a potato shaped rock was obtained. This rock was sitting with the soil even when we started the process to prepare for spot testing the sample, it even got stuck in the test tube at one point.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 16, 2024
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage5483
Genome length (bp)42969
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCCGGAGA
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterFA
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionMarist University
Annotation StatusBeing Annotated (Expected completion by 5/1/2025)
Plaque NotesThe plaques were uniform and clear in the center, fuzzier towards the outside. They were small and abundant.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 5x10^10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload