Mycobacterium phage JerryMouse
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| Detailed Information for Phage JerryMouse | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Bailey Nunn, Zoe Leach |
| Year Found | 2023 |
| Location Found | Boulder, CO United States |
| Finding Institution | University of Colorado Boulder |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.00913 N, 105.26893 W Map |
| Discovery Notes | Found on 9/13/23 at 10:34 AM near recreation center on campus in grassy wet soil near the sidewalk |
| Naming Notes | This phage was very difficult to obtain with many rounds of enrichment and because of that, we are referencing the mouse in Tom and Jerry who is very difficult to catch. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | A single phage with polymorphic plaques had been obtained. The morphologies consist of typical clear plaques with large halos that range from approximately 1mm across to 4mm, the majority falling in between the 2-3mm range. This indicates that the phages are capable of a higher growth rate. Increased growth rate is proportional to increased diffusion, smaller genome, larger burst size, or a more effective absorption rate. The clarity of the plaques establishes that there are lysogens present in that part of the plaque while the turbidity around the edge of the plaque signifies cells that are not fully lysed. This indicates that a temperate phage is present. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 8.54 x 10^10 pfu/mL |
| Pitt Freezer Box# | 178 |
| Pitt Freezer Box Grid# | C7 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
