| Detailed Information for Phage Exile |
| Discovery Information |
| Isolation Host | Arthrobacter sulfureus NRRL B-14730 |
| Former names | Twizzle |
| Found By | Soniya Agarwal |
| Year Found | 2023 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 13°C |
| GPS Coordinates | 45.270408 N, 75.898157 W Map |
| Discovery Notes | Soil was collected from under a young-tree on the evening of September 20th, 2023 in Stittsville, Ottawa. The soil was damp, dark and was collected about eight centimetres from the surface. |
| Naming Notes | Phage was named after one of my favourite songs from the Folklore album by Taylor Swift. Phage also caused lots of trouble during purification and amplification, and thus, deserved to be "exiled". |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 7, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1095 |
| Genome length (bp) | 44132 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGAAGGGGCAT |
| GC Content | 65.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AZ |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Phage is lysogenic, forming plaques with cloudy centres. |
| Other Cluster Members |
|
| Annotating Institution | University of Ottawa |
| Annotation Status | Being Annotated (Expected completion by 5/1/2024) |
| Plaque Notes | Plaque are relatively small in size, and are turbid in the centre and then sharply turn clear at the periphery. Plaques resemble a "bullseye" configuration. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
176 |
| Pitt Freezer Box Grid# |
C4 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
