Streptomyces phage EnochSoames
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Detailed Information for Phage EnochSoames
Discovery Information
Isolation HostStreptomyces coelicolor M145
Found ByZachary Mitchell
Year Found2022
Location FoundOttawa, Canada
Finding InstitutionUniversity of Ottawa
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature16°C
GPS Coordinates45.41421 N, 75.6657 W Map
Discovery NotesIsolated from damp, packed, dark coloured soil collected at the bank of the Rideau River near Hurdman Bridge.
Naming NotesFrom a story by Max Beerbohm. Unfortunately, Soames came too early to see this great phage named after him :-(
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 5, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage18
Genome length (bp)49785
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGGGCAGTGAT
GC Content65.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBD
SubclusterBD1
Cluster Life CycleTemperate
Lysogeny NotesSeems to form lysogen in S. coelicolor.... Despite trying I was unable to isolate it: the lysogenic behaviour disappeared as soon as the bacteria was sporulated.

Achieves full infection in S. avermitilis and S. venezuelae but appears extremely lytic in these two (very clear plaques). No attempt to purify a lysogen has been made in these species.
Other Cluster Members
Annotating InstitutionUniversity of Ottawa
Annotation StatusBeing Annotated (Expected completion by 5/1/2023)
Plaque NotesPlaques are 0.3-0.5 cm in diameter and have an evident bullseye pattern with sharp, clear edges and turbid centres (S. coelicolor).
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

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Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload