| Detailed Information for Phage EnochSoames |
| Discovery Information |
| Isolation Host | Streptomyces coelicolor M145 |
| Found By | Zachary Mitchell |
| Year Found | 2022 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 16°C |
| GPS Coordinates | 45.41421 N, 75.6657 W Map |
| Discovery Notes | Isolated from damp, packed, dark coloured soil collected at the bank of the Rideau River near Hurdman Bridge. |
| Naming Notes | From a story by Max Beerbohm. Unfortunately, Soames came too early to see this great phage named after him :-( |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 5, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 18 |
| Genome length (bp) | 49785 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGGGCAGTGAT |
| GC Content | 65.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BD |
| Subcluster | BD1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Seems to form lysogen in S. coelicolor.... Despite trying I was unable to isolate it: the lysogenic behaviour disappeared as soon as the bacteria was sporulated.
Achieves full infection in S. avermitilis and S. venezuelae but appears extremely lytic in these two (very clear plaques). No attempt to purify a lysogen has been made in these species. |
| Other Cluster Members |
|
| Annotating Institution | University of Ottawa |
| Annotation Status | Being Annotated (Expected completion by 5/1/2024) |
| Plaque Notes | Plaques are 0.3-0.5 cm in diameter and have an evident bullseye pattern with sharp, clear edges and turbid centres (S. coelicolor). |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
154 |
| Pitt Freezer Box Grid# |
C8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
