Mycobacterium phage Kavv
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| Detailed Information for Phage Kavv | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Kyle Vogel and Ainelid Veras |
| Year Found | 2023 |
| Location Found | North Andover, MA United States of America |
| Finding Institution | Merrimack College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 42.666705 N, 71.118827 W Map |
| Discovery Notes | All sampling had taken place about an hour and a half before isolation at approximately 10:45 in the morning. The temperature was 70°F and with air humidity at 93% due to the current rain storms that had previously taken place. There was a slight breeze of 1 mph winds that morning and GPS coordinates (42.6670454° N, -71.1188274° W). The sample sight I was at was a small patch of dirt that was a little grassy with a good amount of moisture and mud. The soil had been distributed with my hands with blue latex gloves on. Sampling had started after just a few inches of digging using my hands. With my hands I initially dug down around 2 to 3 inches into the soil. At the time the soil had been very wet and muddy due to the current weather that had taken place beforehand. After I had collected my sample, I had managed to put around a handful of soil into a clear plastic bag. I had carefully placed my right hand with the soil cupped in it, into the bag that my left hand was holding open to support this action. After I had placed the soil into the plastic bag I had zipped the bag up and was ready to begin isolation and configure these bacteriophages. |
| Naming Notes | Named after the lab partners that discovered this phage. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | In these 3 plaques, obviously each one looks different from each other. In plaque 10-1 you can see there are many spots visible. There are roughly around a hundred of these spots scattered around the plaque in a grayish cloudy color, kind of like the color of a rain cloud. As you can see, the edges of this plaque has a very vibrant tan color surrounding it. In plaque 10-3 this plaque has maybe 30 of these little grayish rain cloudy spots scattered around it. It has noticeably less spots than plaque 10-1 as you can see but still has the vibrant tan ring along the edges. In plaque 10-5 you can see we had only 2 gray spots with the same tan ring and number 1 and 2 circles around them. That is because we will be using these for our next lab isolation. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 182 |
| Pitt Freezer Box Grid# | C1 |
| Available Files | |
| Plaque Picture | Download |
