Gordonia phage Lotus
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Detailed Information for Phage Lotus | |
Discovery Information | |
Isolation Host | Gordonia terrae 3612 |
Former names | Nicholas |
Found By | Meghan Criswell |
Year Found | 2016 |
Location Found | Pittsburgh, PA USA |
Finding Institution | University of Pittsburgh |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 30°C |
GPS Coordinates | Unavailable |
Discovery Notes | Found a cemented in area of soil (supposed to be a flower bed) on the corner of Forbes Avenue and South Bouquet Street in front of the Barcow Law Building on the University of Pittsburgh’s campus along the sidewalks around 3:45 PM on January 9th, 2016. There were very few plants budding in the flowerbed and with the moist environment from the rain that previous night, it could be a sufficient place for bacteria, and even phages, to grow. |
Naming Notes | After a great actor, Nicholas Cage. He starred in a movie titled "Frozen Ground" in 2013, which is what I had to dig through to collect soil samples this winter semester. It was a "National Treasure" when I finally found a phage. |
Sequencing Information | |
Sequencing Complete? | No |
Genome length (bp) | Unknown |
Character of genome ends | Unknown |
Fasta file available? | No |
Characterization | |
Cluster | Unclustered |
Subcluster | -- |
Annotating Institution | Unknown or unassigned |
Annotation Status | Not sequenced |
Plaque Notes | It was fairly obvious I had a phage from the results of my spot test after enriching my soil with the huge clearing in the middle of the plate. While serial diluting, I found multiple sized plaques, so I am currently working on purifying my plaque to only one species. Results to follow. |
Has been Phamerated? | No |
Publication Info | |
Uploaded to GenBank? | No |
GenBank Accession | None yet |
Refseq Number | None yet |
Archiving Info | |
Archiving status | Archived |
SEA Lysate Titer | 3.77 x 10^10 |
Date of SEA Lysate Titering | Mar 30, 2016 |
Pitt Freezer Box# | 3 |
Pitt Freezer Box Grid# | E4 |
Available Files | |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |