Gordonia phage Lotus
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Detailed Information for Phage Lotus
Discovery Information
Isolation HostGordonia terrae 3612
Former namesNicholas
Found ByMeghan Criswell
Year Found2016
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS CoordinatesUnavailable
Discovery NotesFound a cemented in area of soil (supposed to be a flower bed) on the corner of Forbes Avenue and South Bouquet Street in front of the Barcow Law Building on the University of Pittsburgh’s campus along the sidewalks around 3:45 PM on January 9th, 2016.
There were very few plants budding in the flowerbed and with the moist environment from the rain that previous night, it could be a sufficient place for bacteria, and even phages, to grow.
Naming NotesAfter a great actor, Nicholas Cage. He starred in a movie titled "Frozen Ground" in 2013, which is what I had to dig through to collect soil samples this winter semester. It was a "National Treasure" when I finally found a phage.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesIt was fairly obvious I had a phage from the results of my spot test after enriching my soil with the huge clearing in the middle of the plate. While serial diluting, I found multiple sized plaques, so I am currently working on purifying my plaque to only one species. Results to follow.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 3.77 x 10^10
Date of SEA Lysate Titering Mar 30, 2016
Pitt Freezer Box# 3
Pitt Freezer Box Grid# E4
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload