Mycobacterium phage Lulu12
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| Detailed Information for Phage Lulu12 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Emily K Gough |
| Year Found | 2012 |
| Location Found | Columbus, OH USA |
| Finding Institution | The Ohio State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.002942 N, 83.027672 W Map |
| Discovery Notes | The soil sample was taken from the Ohio State Chadwick Arboretum and Learning Gardens near the Japanese False Cypress, "Caramel" Coral Bells, and the "Newport" Cherry Plum plants. Soil was moist and taken from 3cm deep. |
| Naming Notes | Lulu is a childhood nickname and the phage was found in '12. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaques tend to be 5mm in diameter with a clear center of 2mm surrounded by a cloudy ring. I noticed that the greater the concentration of phage, the smaller the plaques became, probably due to greater competition for host and space. I also noticed that given an incubation time of more than 24 hours, plaques grew larger and exhibited a bulls-eye pattern. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | 2012OHSUlulu12EKG |
| SEA Lysate Titer | 4.4x10^10 |
| Date of SEA Lysate Titering | Oct 29, 2012 |
| Pitt Freezer Box# | 38 |
| Pitt Freezer Box Grid# | 33 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
